Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613131811_axwh CCCTAAGGAGACGTCAGGGTGCCGTCGGGCTG(+AGGACA)GATCCTAGGTGGACACTTGGTACCTCTCTGT NGG 10 8 17 8 24 0 100 5 10
    240613131809_1orf CAGCAACAGAGAGGTACCAAGTGTCCACCTAG(+AGGACA)GATCCAGCCCGACGGCACCCTGACGTCTCCT NGG 10 8 17 8 24 0 100 5 10
    240613131806_peqk CCTGGCCTACAATCCCTTGTCAGGCGTCCAGG(+AGGACA)GTACCACACTGAGAACAGCTGGCATTTGGAT NGG 10 8 17 8 24 0 100 5 10
    240613131805_md1u GAACCATCCAAATGCCAGCTGTTCTCAGTGTG(+AGGACA)GTACCCTGGACGCCTGACAAGGGATTGTAGG NGG 10 8 17 8 24 0 100 5 10
    240613131804_r9m1 TCCATGCTCTCCCAATAAACATGTTGCAGAAG(+AGGACA)GATCCTGTTGTGGCATCTTACTTGCTGGCGA NGG 10 8 17 8 24 0 100 5 10
    240613131803_idqu CCCACTCGCCAGCAAGTAAGATGCCACAACAG(+AGGACA)GATCCTTCTGCAACATGTTTATTGGGAGAGC NGG 10 8 17 8 24 0 100 5 10
    240613131801_4vu6 TCCATGCTCTCCCAATAAACATGTTGCAGAAG(+AGGACA)GATCCTGTTGTGGCATCTTACTTGCTGGCGA NGG 10 8 17 8 24 0 100 5 10
    240613131800_lfag CCCACTCGCCAGCAAGTAAGATGCCACAACAG(+AGGACA)GATCCTTCTGCAACATGTTTATTGGGAGAGC NGG 10 8 17 8 24 0 100 5 10
    240613131759_thj1 AAACTCTAGCAAGTGGGAGCCTATCTTTGAAG(+AGGACA)GTACCCTGCTGCTCACACTGCTTAGCCCACG NGG 10 8 17 8 24 0 100 5 10
    240613131757_7n4f GTGGGCGTGGGCTAAGCAGTGTGAGCAGCAGG(+AGGACA)GTACCTTCAAAGATAGGCTCCCACTTGCTAG NGG 10 8 17 8 24 0 100 5 10