Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613131709_0yn6 GCATGTATGTCTGTGTTTCAGTAGCATGCTTG(+AGGACA)GTACCCAAGGAAGCCAGAAAAAGGCATTAGA NGG 10 8 17 8 24 0 100 5 10
    240613131707_d3y5 GGGCATCTAATGCCTTTTTCTGGCTTCCTTGG(+AGGACA)GTACCAAGCATGCTACTGAAACACAGACATA NGG 10 8 17 8 24 0 100 5 10
    240613131704_i2j2 GCTCACGGCCATTGCAGCTCTTCCTTCCAGGG(+AGGACA)GATCCAACTCCCTCTTGGGCCTCCACTGACA NGG 10 8 17 8 24 0 100 5 10
    240613131703_vxbm CTTTGTGTCAGTGGAGGCCCAAGAGGGAGTTG(+AGGACA)GATCCCCTGGAAGGAAGAGCTGCAATGGCCG NGG 10 8 17 8 24 0 100 5 10
    240613131701_9dnx CGGGGACTGTCCCTGCCTCTTGCCTGCCTGTG(+AGGACA)GATCCCTTTCCACTAACACACATTTTAAAAA NGG 10 8 17 8 24 0 100 5 10
    240613131700_w7hh TCAGCTTTTTAAAATGTGTGTTAGTGGAAAGG(+AGGACA)GATCCACAGGCAGGCAAGAGGCAGGGACAGT NGG 10 8 17 8 24 0 100 5 10
    240613131658_ncwy GGAGGAGAAGGCAAGGAACAATGGACAACTGG(+AGGACA)GATCCATTGAGCCCCAGGACCAGTGTGCCCC NGG 10 8 17 8 24 0 100 5 10
    240613131657_ahol GACATGGGGCACACTGGTCCTGGGGCTCAATG(+AGGACA)GATCCCAGTTGTCCATTGTTCCTTGCCTTCT NGG 10 8 17 8 24 0 100 5 10
    240613131654_7xoe ACTGCCCACCTGATGCCCACGAGGCCCCCCAG(+AGGACA)GATCCAAATGAACTGGGCATGATGGCCCCTG NGG 10 8 17 8 24 0 100 5 10
    240613131652_2c1e ACAGGCAGGGGCCATCATGCCCAGTTCATTTG(+AGGACA)GATCCTGGGGGGCCTCGTGGGCATCAGGTGG NGG 10 8 17 8 24 0 100 5 10