Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613131635_930w GAGACCCCGAGGGAGGTGAGCTTGAAGTGAGG(+AGGACA)GTACCATCCAAGGTACTCCAGGACCCTCTCC NGG 10 8 17 8 24 0 100 5 10
    240613131633_y29r GACCAGGAGAGGGTCCTGGAGTACCTTGGATG(+AGGACA)GTACCCTCACTTCAAGCTCACCTCCCTCGGG NGG 10 8 17 8 24 0 100 5 10
    240613131631_4wuu GGACCGGCCTTTACTGAGCTGTCACACAGTGG(+AGGACA)GTACCTCTAGGTGCTTTTGGGATGGCAGGTA NGG 10 8 17 8 24 0 100 5 10
    240613131630_7ru7 AGAGATACCTGCCATCCCAAAAGCACCTAGAG(+AGGACA)GTACCCACTGTGTGACAGCTCAGTAAAGGCC NGG 10 8 17 8 24 0 100 5 10
    240613131629_mlmn CAGGGGATTTCATCAAGAATCCCAACTATTTG(+AGGACA)GTACCAGAGCCAAGCAAATGCAAGCAGAACA NGG 10 8 17 8 24 0 100 5 10
    240613131628_955p CCAAGTGTTCTGCTTGCATTTGCTTGGCTCTG(+AGGACA)GTACCAAATAGTTGGGATTCTTGATGAAATC NGG 10 8 17 8 24 0 100 5 10
    240613131626_jtha GAGCTGCTGCCACACTCGGGCCCAGCGAGGAG(+AGGACA)GATCCGGGAAAGGCACCTCGGAACTCAGCCA NGG 10 8 17 8 24 0 100 5 10
    240613131624_kwq9 CTTTGTGGCTGAGTTCCGAGGTGCCTTTCCCG(+AGGACA)GATCCTCCTCGCTGGGCCCGAGTGTGGCAGC NGG 10 8 17 8 24 0 100 5 10
    240613131622_es4v GTCTTCTCCCAATCACAGTTCCAGGGGCCAGG(+AGGACA)GTACCTCCAGAGGACTTCTCAAGCTGGTTGG NGG 10 8 17 8 24 0 100 5 10
    240613131620_m4ae GAAACCCAACCAGCTTGAGAAGTCCTCTGGAG(+AGGACA)GTACCCTGGCCCCTGGAACTGTGATTGGGAG NGG 10 8 17 8 24 0 100 5 10