Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613131533_vspz CAGGCCAGGACCCACACAACTGATGGGGGCAG(+AGGACA)GATCCCCAGTGATGGGTCCAGAGTGGGAATC NGG 10 8 17 8 24 0 100 5 10
    240613131530_afua GACTTGATTCCCACTCTGGACCCATCACTGGG(+AGGACA)GATCCTGCCCCCATCAGTTGTGTGGGTCCTG NGG 10 8 17 8 24 0 100 5 10
    240613131529_xtl5 GGTCTCCCGAGAGCATGCCGAGTGTCCTGAGG(+AGGACA)GATCCTCGGCTAGCCCCCATCTTCTACTTGA NGG 10 8 17 8 24 0 100 5 10
    240613131527_kxyj AGCCTTCAAGTAGAAGATGGGGGCTAGCCGAG(+AGGACA)GATCCCTCAGGACACTCGGCATGCTCTCGGG NGG 10 8 17 8 24 0 100 5 10
    240613131525_lq2b GGAGGGAACCTTGGCAGCACTGGGCCTGTAGG(+AGGACA)GATCCAGAGTTCTCAGGCAATAGAAGTGATC NGG 10 8 17 8 24 0 100 5 10
    240613131524_829z ACCCAGATCACTTCTATTGCCTGAGAACTCTG(+AGGACA)GATCCCTACAGGCCCAGTGCTGCCAAGGTTC NGG 10 8 17 8 24 0 100 5 10
    240613131522_p3n3 GAGGGTGGTCTGTCGCATCACCACATTAGTGG(+AGGACA)GTACCTGACAGTTACCTCATTGGCAGCCAAG NGG 10 8 17 8 24 0 100 5 10
    240613131521_8gyy CTCACCTTGGCTGCCAATGAGGTAACTGTCAG(+AGGACA)GTACCCACTAATGTGGTGATGCGACAGACCA NGG 10 8 17 8 24 0 100 5 10
    240613131519_p03l CTGCCCCTCTGACTCCTGCCCAGAAGTGGTGG(+AGGACA)GTACCCCCCCACCCCGTGCCATTCATTGTCC NGG 10 8 17 8 24 0 100 5 10
    240613131517_jfr5 GGGTGGGACAATGAATGGCACGGGGTGGGGGG(+AGGACA)GTACCCACCACTTCTGGGCAGGAGTCAGAGG NGG 10 8 17 8 24 0 100 5 10