Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613131436_8c9x CTTGAAAAACAGAGGCTTTGTCGTTCTCATAG(+AGGACA)GATCCACAGTGTCTGAGCTATCCACGCTGAC NGG 10 8 17 8 24 0 100 5 10
    240613131435_son5 TTGGTGTCAGCGTGGATAGCTCAGACACTGTG(+AGGACA)GATCCTATGAGAACGACAAAGCCTCTGTTTT NGG 10 8 17 8 24 0 100 5 10
    240613131433_iat4 CCCACATGCCTGGTGCATGGCCTGCTCCCTGG(+AGGACA)GATCCCCCTGGCTGCATGCTTACTGCAGCGC NGG 10 8 17 8 24 0 100 5 10
    240613131430_6fat GCATGGCGCTGCAGTAAGCATGCAGCCAGGGG(+AGGACA)GATCCCAGGGAGCAGGCCATGCACCAGGCAT NGG 10 8 17 8 24 0 100 5 10
    240613131429_5psp CCCCACCCCACTCACCCAGTGTCTGGGCTTAG(+AGGACA)GATCCTCAGGCAAGGCCCCTGGGGTGGGGCT NGG 10 8 17 8 24 0 100 5 10
    240613131427_uu04 CCAGGAGCCCCACCCCAGGGGCCTTGCCTGAG(+AGGACA)GATCCTAAGCCCAGACACTGGGTGAGTGGGG NGG 10 8 17 8 24 0 100 5 10
    240613131425_6vnw ATCATTCACTGTGACACCCCAGCCCTCCAAGG(+AGGACA)GTACCCCGGGAGACCACGTGACTCTCTCTCT NGG 10 8 17 8 24 0 100 5 10
    240613131422_u58s GAGAGAGAGAGAGAGTCACGTGGTCTCCCGGG(+AGGACA)GTACCCTTGGAGGGCTGGGGTGTCACAGTGA NGG 10 8 17 8 24 0 100 5 10
    240613131420_jx9f GTGCTATCCAGACCTTCTGTCTCCTCTTCAGG(+AGGACA)GTACCAAACAGACACCGTCACTGTGGCAGTC NGG 10 8 17 8 24 0 100 5 10
    240613131419_kwx1 CTCAGGACTGCCACAGTGACGGTGTCTGTTTG(+AGGACA)GTACCCTGAAGAGGAGACAGAAGGTCTGGAT NGG 10 8 17 8 24 0 100 5 10