Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613131347_6rzd GGTGTCAGCAGTTGGAGCAGCCTGGAGTTCTG(+AGGACA)GATCCTGGCTCCTAGGTGGTTTTCACATCAG NGG 10 8 17 8 24 0 100 5 10
    240613131345_vhph AGAGGCTGATGTGAAAACCACCTAGGAGCCAG(+AGGACA)GATCCAGAACTCCAGGCTGCTCCAACTGCTG NGG 10 8 17 8 24 0 100 5 10
    240613131343_a1h3 TGTGTATGCAAAAACTCCTGTCCACATGGTGG(+AGGACA)GTACCCCACGGAAGACAGCTTTCTTATTTGA NGG 10 8 17 8 24 0 100 5 10
    240613131341_gflq GTTTTTCAAATAAGAAAGCTGTCTTCCGTGGG(+AGGACA)GTACCCACCATGTGGACAGGAGTTTTTGCAT NGG 10 8 17 8 24 0 100 5 10
    240613131340_mzcw CACACCACATCATTCAGTCAGACTGGTTTTTG(+AGGACA)GTACCTAAGTATTTGTGTTCAAGTAACCTTT NGG 10 8 17 8 24 0 100 5 10
    240613131339_wegi GCAGTAAAGGTTACTTGAACACAAATACTTAG(+AGGACA)GTACCAAAAACCAGTCTGACTGAATGATGTG NGG 10 8 17 8 24 0 100 5 10
    240613131337_lvbl GGGACTTTGACAGATTGTCTTGTAGCAGGGAG(+AGGACA)GATCCAGCCCAGCTATGGGCCTGGGATGCCA NGG 10 8 17 8 24 0 100 5 10
    240613131335_7l9c CTCCCTGGCATCCCAGGCCCATAGCTGGGCTG(+AGGACA)GATCCTCCCTGCTACAAGACAATCTGTCAAA NGG 10 8 17 8 24 0 100 5 10
    240613131333_xfz8 GAGACCCACCTGTCACCGTGTGTCATCAAGGG(+AGGACA)GATCCGGTGGGGAAAAGAAGGGGAAACACAG NGG 10 8 17 8 24 0 100 5 10
    240613131331_5aod CTCACCTGTGTTTCCCCTTCTTTTCCCCACCG(+AGGACA)GATCCCCTTGATGACACACGGTGACAGGTGG NGG 10 8 17 8 24 0 100 5 10