Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613131315_qm1d GAAGGCAGGTCCCCAGTTGTTGGGCCAGCAGG(+AGGACA)GTACCTTCCTTCTTCTGCAGACTGCGATAGT NGG 10 8 17 8 24 0 100 5 10
    240613131313_28h9 GTCACACTATCGCAGTCTGCAGAAGAAGGAAG(+AGGACA)GTACCCTGCTGGCCCAACAACTGGGGACCTG NGG 10 8 17 8 24 0 100 5 10
    240613131312_3v7y ACGCCTTTAGTCTCCGGATGTCTCAAATTATG(+AGGACA)GATCCTGAGCGCAGCCGTCTGCTCTTTCATG NGG 10 8 17 8 24 0 100 5 10
    240613131311_hao2 TTATGCATGAAAGAGCAGACGGCTGCGCTCAG(+AGGACA)GATCCATAATTTGAGACATCCGGAGACTAAA NGG 10 8 17 8 24 0 100 5 10
    240613131309_wq7v CCTGTGTCTCAGGGACGCCTCTACTTTCTTGG(+AGGACA)GATCCGGGACCGTTCTCAGAGGCACTGGGGA NGG 10 8 17 8 24 0 100 5 10
    240613131307_htih TTCCGTCCCCAGTGCCTCTGAGAACGGTCCCG(+AGGACA)GATCCCAAGAAAGTAGAGGCGTCCCTGAGAC NGG 10 8 17 8 24 0 100 5 10
    240613131305_c3uk CAGCAAGATGCTCCTGATGCCCTTTGGTCCAG(+AGGACA)GATCCACTGCCTCCTTGTTCTAGAGGCTCAG NGG 10 8 17 8 24 0 100 5 10
    240613131304_2xum GATGTCTGAGCCTCTAGAACAAGGAGGCAGTG(+AGGACA)GATCCTGGACCAAAGGGCATCAGGAGCATCT NGG 10 8 17 8 24 0 100 5 10
    240613131302_loet CCGCATGATGGCTGGGTCTGAAATCGCAAATG(+AGGACA)GATCCAGCCTGATGTCCTCGCAGTTGCACAT NGG 10 8 17 8 24 0 100 5 10
    240613131301_o85f CCCACATGTGCAACTGCGAGGACATCAGGCTG(+AGGACA)GATCCATTTGCGATTTCAGACCCAGCCATCA NGG 10 8 17 8 24 0 100 5 10