Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613131230_mmyq AGTTTATCTCTTCCTAGTGATCAGCGAGGTTG(+AGGACA)GATCCCCAGGCTCTGTGAATGGCCCAACCGT NGG 10 8 17 8 24 0 100 5 10
    240613131228_2nu1 GGGGTACGGTTGGGCCATTCACAGAGCCTGGG(+AGGACA)GATCCAACCTCGCTGATCACTAGGAAGAGAT NGG 10 8 17 8 24 0 100 5 10
    240613131226_8yic CAGAAGTAAAAGCAGGCCCAAGAGCAAAGGAG(+AGGACA)GTACCAGGAGGCCCCCTGCTGGTGAGCTTGA NGG 10 8 17 8 24 0 100 5 10
    240613131224_gacu ATGACTCAAGCTCACCAGCAGGGGGCCTCCTG(+AGGACA)GTACCTCCTTTGCTCTTGGGCCTGCTTTTAC NGG 10 8 17 8 24 0 100 5 10
    240613131223_4mn5 CACCAGTGTTCGGGTGGAAGCATAGGAAACAG(+AGGACA)GATCCCGCAGTGGCAAGGTCAGGCCACCTGC NGG 10 8 17 8 24 0 100 5 10
    240613131221_2na4 CCCAGGCAGGTGGCCTGACCTTGCCACTGCGG(+AGGACA)GATCCTGTTTCCTATGCTTCCACCCGAACAC NGG 10 8 17 8 24 0 100 5 10
    240613131220_08a1 TCCAAAATGTGAATAACTTGTCAGAGCAGCTG(+AGGACA)GTACCCCTGATCGGCTGGGTCTGCTTCCGGT NGG 10 8 17 8 24 0 100 5 10
    240613131218_clqs TAGCAACCGGAAGCAGACCCAGCCGATCAGGG(+AGGACA)GTACCAGCTGCTCTGACAAGTTATTCACATT NGG 10 8 17 8 24 0 100 5 10
    240613131217_kiyc ACGAGGTGGCTGGAGGGTGCTCTCAGCTGCAG(+AGGACA)GTACCAGACCCAGGACAGGAAATGTCAGCCA NGG 10 8 17 8 24 0 100 5 10
    240613131216_7v9d GCTGATGGCTGACATTTCCTGTCCTGGGTCTG(+AGGACA)GTACCTGCAGCTGAGAGCACCCTCCAGCCAC NGG 10 8 17 8 24 0 100 5 10