Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613131158_dmz3 TCTGGCATTTCATGTTACTGCAGGCCTTTGAG(+AGGACA)GTACCTCAATGTGTAAGATATTTCTGTGGGG NGG 10 8 17 8 24 0 100 5 10
    240613131157_h2q7 TTAAGCCCCACAGAAATATCTTACACATTGAG(+AGGACA)GTACCTCAAAGGCCTGCAGTAACATGAAATG NGG 10 8 17 8 24 0 100 5 10
    240613131156_3rku ATACTGAAACAGACTTCCAACAGGAAGGCACG(+AGGACA)GTACCTGAGAGCAAGTGTTTCTGAATAGAAC NGG 10 8 17 8 24 0 100 5 10
    240613131155_zccm GTCCAGTTCTATTCAGAAACACTTGCTCTCAG(+AGGACA)GTACCGTGCCTTCCTGTTGGAAGTCTGTTTC NGG 10 8 17 8 24 0 100 5 10
    240613131153_6ac0 TAAATGACAGTGTGTAACTATCTGTAACTGGG(+AGGACA)GATCCCATGCCCTTTTCTGACCTTCAAGGTC NGG 10 8 17 8 24 0 100 5 10
    240613131151_k1j9 TTGGTGACCTTGAAGGTCAGAAAAGGGCATGG(+AGGACA)GATCCCCAGTTACAGATAGTTACACACTGTC NGG 10 8 17 8 24 0 100 5 10
    240613131150_ibnq GCCGTTAGACTGCCTGCCTTTCAGAGGTCTGG(+AGGACA)GATCCTGGCTCCCCACCCCCAAGTGCGAGAC NGG 10 8 17 8 24 0 100 5 10
    240613131148_omxx AAATGGTCTCGCACTTGGGGGTGGGGAGCCAG(+AGGACA)GATCCCAGACCTCTGAAAGGCAGGCAGTCTA NGG 10 8 17 8 24 0 100 5 10
    240613131146_5hmx TTAGGAACAAGGCAGAGAGCACAGTCGCTCTG(+AGGACA)GATCCACCCTAAAAAGCCTGCTGACGTGGAG NGG 10 8 17 8 24 0 100 5 10
    240613131145_n4s8 TGTCCCTCCACGTCAGCAGGCTTTTTAGGGTG(+AGGACA)GATCCAGAGCGACTGTGCTCTCTGCCTTGTT NGG 10 8 17 8 24 0 100 5 10