Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613131143_j3bf TCCTCATGTAGCTCACAAGGGCTGGTGTGGAG(+AGGACA)GATCCGCCTTCTTGCTGGAGAACCCAGCCTC NGG 10 8 17 8 24 0 100 5 10
    240613131142_gha3 TAGGGGAGGCTGGGTTCTCCAGCAAGAAGGCG(+AGGACA)GATCCTCCACACCAGCCCTTGTGAGCTACAT NGG 10 8 17 8 24 0 100 5 10
    240613131140_8v1c GGCAGAGATGGCTCCATGTTTTACAAACTATG(+AGGACA)GATCCCTGGGCAGAGTTCAGGTGTCCCTGTA NGG 10 8 17 8 24 0 100 5 10
    240613131138_9j5t AAGGATACAGGGACACCTGAACTCTGCCCAGG(+AGGACA)GATCCATAGTTTGTAAAACATGGAGCCATCT NGG 10 8 17 8 24 0 100 5 10
    240613131137_s4ba GAGGCATGTCAGCTACCAGTTTCTCCACAGAG(+AGGACA)GATCCAAGAAGTACATCAAAGATGGTCTACC NGG 10 8 17 8 24 0 100 5 10
    240613131135_v714 GAGATGGTAGACCATCTTTGATGTACTTCTTG(+AGGACA)GATCCTCTGTGGAGAAACTGGTAGCTGACAT NGG 10 8 17 8 24 0 100 5 10
    240613131134_eyv1 CAGTGAGTGCCAGCGGTGCTGGAGCTAGAGCG(+AGGACA)GATCCTGAAGGCAGTGACATTGGGACAGTCA NGG 10 8 17 8 24 0 100 5 10
    240613131133_cr25 GACCCTGACTGTCCCAATGTCACTGCCTTCAG(+AGGACA)GATCCGCTCTAGCTCCAGCACCGCTGGCACT NGG 10 8 17 8 24 0 100 5 10
    240613131131_q15d TAAGAAGAGTCTCCCCTGAAGTGTTTGTGGTG(+AGGACA)GTACCTGTGTGCAGAAGGGTGTGTCGCCACT NGG 10 8 17 8 24 0 100 5 10
    240613131130_0vvl TGCCAAGTGGCGACACACCCTTCTGCACACAG(+AGGACA)GTACCACCACAAACACTTCAGGGGAGACTCT NGG 10 8 17 8 24 0 100 5 10