Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613131243_rzcv CCTGAGCCTCCCTCGGGCACCTGTTGCTTCTG(+AGGACA)GATCCGAATAATCCAGGAGGCTCCCCACCCT NGG 10 8 17 8 24 0 100 5 10
    240613131242_lucn GCCACAGGGTGGGGAGCCTCCTGGATTATTCG(+AGGACA)GATCCAGAAGCAACAGGTGCCCGAGGGAGGC NGG 10 8 17 8 24 0 100 5 10
    240613131241_4nrb GCCACACACATACCGAGACTCTGTGAATGATG(+AGGACA)GTACCACTCTACCTAGGACAGGAGAGACCGA NGG 10 8 17 8 24 0 100 5 10
    240613131240_j3k8 CTTTGTCGGTCTCTCCTGTCCTAGGTAGAGTG(+AGGACA)GTACCATCATTCACAGAGTCTCGGTATGTGT NGG 10 8 17 8 24 0 100 5 10
    240613131238_1s5f CCCCGCCTTCAGGAAGACTTCCAGGACTGACG(+AGGACA)GTACCCAGTCTCGGCGCCCTCCTCCCAGGAG NGG 10 8 17 8 24 0 100 5 10
    240613131237_qv9s AAGGGCTCCTGGGAGGAGGGCGCCGAGACTGG(+AGGACA)GTACCGTCAGTCCTGGAAGTCTTCCTGAAGG NGG 10 8 17 8 24 0 100 5 10
    240613131235_pyw5 CTCAACCAATGAAAAGTCAAAAGTCCTCCTAG(+AGGACA)GATCCTGTGTAGCATTCCTGGGCCTGTTCCA NGG 10 8 17 8 24 0 100 5 10
    240613131234_9lfo GAGAATGGAACAGGCCCAGGAATGCTACACAG(+AGGACA)GATCCTAGGAGGACTTTTGACTTTTCATTGG NGG 10 8 17 8 24 0 100 5 10
    240613131232_8nl4 TGCCGGCTTTCAGCTTGGCTGGGCTGGGCTGG(+AGGACA)GATCCTGAACTGTATCCATTATCCCTGGGTG NGG 10 8 17 8 24 0 100 5 10
    240613131231_a32y TTTCGCACCCAGGGATAATGGATACAGTTCAG(+AGGACA)GATCCCAGCCCAGCCCAGCCAAGCTGAAAGC NGG 10 8 17 8 24 0 100 5 10