Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613131417_20ce TCTTGCAAGGGACTCTTCCCCCTGCCCCTCAG(+AGGACA)GATCCAAAAGGCCCAGGCTGAGGAAACTCAT NGG 10 8 17 8 24 0 100 5 10
    240613131416_ycdi CCTCCATGAGTTTCCTCAGCCTGGGCCTTTTG(+AGGACA)GATCCTGAGGGGCAGGGGGAAGAGTCCCTTG NGG 10 8 17 8 24 0 100 5 10
    240613131414_peum ACAACATAGTGGCTCACAACCATCTGTAATGG(+AGGACA)GATCCGATGCCCGCTTCTGGTGTGTCTGAAG NGG 10 8 17 8 24 0 100 5 10
    240613131413_5bou GCTGTCTTCAGACACACCAGAAGCGGGCATCG(+AGGACA)GATCCCATTACAGATGGTTGTGAGCCACTAT NGG 10 8 17 8 24 0 100 5 10
    240613131412_4mk7 GGCCCGGGGCTCCTATTCTTATGTGGCTGCAG(+AGGACA)GATCCTCTGGAAATGACTATGATTTAATGGC NGG 10 8 17 8 24 0 100 5 10
    240613131410_fwrh GCTGAGCCATTAAATCATAGTCATTTCCAGAG(+AGGACA)GATCCTGCAGCCACATAAGAATAGGAGCCCC NGG 10 8 17 8 24 0 100 5 10
    240613131408_dznc TCTCAAGACTGCAGCCACGTAAACTTCCTCTG(+AGGACA)GATCCTAAGTTACTATATAAACGGATCTTTA NGG 10 8 17 8 24 0 100 5 10
    240613131406_qxoe GGTTTTAAAGATCCGTTTATATAGTAACTTAG(+AGGACA)GATCCAGAGGAAGTTTACGTGGCTGCAGTCT NGG 10 8 17 8 24 0 100 5 10
    240613131405_6qpo CTATCTTTTCCTAAAGGCAGTTTCCCTGCTAG(+AGGACA)GATCCCTAAAGTGATTATTGAAGAAAATACA NGG 10 8 17 8 24 0 100 5 10
    240613131403_tfgu GCTTCTGTATTTTCTTCAATAATCACTTTAGG(+AGGACA)GATCCTAGCAGGGAAACTGCCTTTAGGAAAA NGG 10 8 17 8 24 0 100 5 10