Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613131619_z0ab TATACTTGGGGACAGTTACAAGGGAGTCACAG(+AGGACA)GTACCCTGTCCATGTCTGCGGCTGTGGGCAG NGG 10 8 17 8 24 0 100 5 10
    240613131617_0mrd GCAGGCTGCCCACAGCCGCAGACATGGACAGG(+AGGACA)GTACCTGTGACTCCCTTGTAACTGTCCCCAA NGG 10 8 17 8 24 0 100 5 10
    240613131616_gm5w CAAGTCAGAATTCCTGGTAGGGGCGGTTTCAG(+AGGACA)GATCCCACAGAAATGAAGGGACACAGTCTTC NGG 10 8 17 8 24 0 100 5 10
    240613131615_ak62 TGGGAGAAGACTGTGTCCCTTCATTTCTGTGG(+AGGACA)GATCCTGAAACCGCCCCTACCAGGAATTCTG NGG 10 8 17 8 24 0 100 5 10
    240613131612_ems0 CATCCAGGCCCCCAATCTTTTATATTGAAGGG(+AGGACA)GATCCAACTTCCAGGATGAAAGGCAGAGAGC NGG 10 8 17 8 24 0 100 5 10
    240613131611_417r ACCAGGCTCTCTGCCTTTCATCCTGGAAGTTG(+AGGACA)GATCCCCTTCAATATAAAAGATTGGGGGCCT NGG 10 8 17 8 24 0 100 5 10
    240613131610_hmi9 GCTGAGCAAGAAAGGCTGTCTGAGCCATGGTG(+AGGACA)GATCCCCAGGGAGGGAATGTCCCCTCCCCCC NGG 10 8 17 8 24 0 100 5 10
    240613131607_8gci GGGCAGGGGGGAGGGGACATTCCCTCCCTGGG(+AGGACA)GATCCACCATGGCTCAGACAGCCTTTCTTGC NGG 10 8 17 8 24 0 100 5 10
    240613131606_jfxh GCCTACTTCTGGATCACCCTCATCCAGTGCTG(+AGGACA)GTACCCCACAGCACTAAATCCAGGAACCCTG NGG 10 8 17 8 24 0 100 5 10
    240613131604_6te2 AAGCCCAGGGTTCCTGGATTTAGTGCTGTGGG(+AGGACA)GTACCAGCACTGGATGAGGGTGATCCAGAAG NGG 10 8 17 8 24 0 100 5 10