Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613131726_idjr CCAGCCTGCCTTGGCAAAGCCAGGGCCGCTGG(+AGGACA)GATCCGGTGGCAAAGCCAGCTAGCAGCGGCC NGG 10 8 17 8 24 0 100 5 10
    240613131723_ahf6 CACCTGGCCGCTGCTAGCTGGCTTTGCCACCG(+AGGACA)GATCCCAGCGGCCCTGGCTTTGCCAAGGCAG NGG 10 8 17 8 24 0 100 5 10
    240613131721_njmz CCAACTTCAGCCCATTCACGCCCCTGCCTTTG(+AGGACA)GTACCAGCTACTTTCTCCACCCACCCAACTC NGG 10 8 17 8 24 0 100 5 10
    240613131720_qq60 AAGGGGAGTTGGGTGGGTGGAGAAAGTAGCTG(+AGGACA)GTACCAAAGGCAGGGGCGTGAATGGGCTGAA NGG 10 8 17 8 24 0 100 5 10
    240613131718_121c AGATTGGGGCAGTGGGACAAGCTATAATCCTG(+AGGACA)GTACCCAGGAGGCAGTGGTATGGAGATTTTG NGG 10 8 17 8 24 0 100 5 10
    240613131716_8nnw GACCACAAAATCTCCATACCACTGCCTCCTGG(+AGGACA)GTACCAGGATTATAGCTTGTCCCACTGCCCC NGG 10 8 17 8 24 0 100 5 10
    240613131715_6eer TGCAAAACAAAACAAAACAAAACAAAACAAAG(+AGGACA)GATCCTGTATTGGCAGAGATGGTGGTAAAGA NGG 10 8 17 8 24 0 100 5 10
    240613131714_q8en ATGCATCTTTACCACCATCTCTGCCAATACAG(+AGGACA)GATCCTTTGTTTTGTTTTGTTTTGTTTTGTT NGG 10 8 17 8 24 0 100 5 10
    240613131712_8o7s CCAGTTGTAATTGCTTTGAACCAAGCACCTCG(+AGGACA)GATCCTGACTACTTTAAACACTGAGCTCCAG NGG 10 8 17 8 24 0 100 5 10
    240613131710_oov3 TACCACTGGAGCTCAGTGTTTAAAGTAGTCAG(+AGGACA)GATCCGAGGTGCTTGGTTCAAAGCAATTACA NGG 10 8 17 8 24 0 100 5 10