Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613131823_nqpd TCCTCCACAAATAAACGTAATTAAATCTAAAG(+AGGACA)GTACCCTGAGATTTCTGTATAGGAAACAACT NGG 10 8 17 8 24 0 100 5 10
    240613131821_7u7p AAAACAGTTGTTTCCTATACAGAAATCTCAGG(+AGGACA)GTACCTTTAGATTTAATTACGTTTATTTGTG NGG 10 8 17 8 24 0 100 5 10
    240613131820_o2nk AGGTTTCTAGAAACACGCAGACACTGAGAATG(+AGGACA)GTACCCAGGGTCCTGCTCTTTACCACAGGTT NGG 10 8 17 8 24 0 100 5 10
    240613131817_813j GCATGAACCTGTGGTAAAGAGCAGGACCCTGG(+AGGACA)GTACCATTCTCAGTGTCTGCGTGTTTCTAGA NGG 10 8 17 8 24 0 100 5 10
    240613131816_cyiq AGAGGTTAGATGTTCTTGGCCTGCAGGGACAG(+AGGACA)GTACCTATAGTGTTGGCATAGTCAGGTTTCC NGG 10 8 17 8 24 0 100 5 10
    240613131814_72cd TGCTGGGAAACCTGACTATGCCAACACTATAG(+AGGACA)GTACCTGTCCCTGCAGGCCAAGAACATCTAA NGG 10 8 17 8 24 0 100 5 10
    240613131813_a0gv TGCCGTCGGGCTGGATCCTAGGTGGACACTTG(+AGGACA)GTACCTCTCTGTTGCTGTTTCTTGGAGAGCC NGG 10 8 17 8 24 0 100 5 10
    240613131812_bv0r AGCTGGGCTCTCCAAGAAACAGCAACAGAGAG(+AGGACA)GTACCAAGTGTCCACCTAGGATCCAGCCCGA NGG 10 8 17 8 24 0 100 5 10
    240613131811_axwh CCCTAAGGAGACGTCAGGGTGCCGTCGGGCTG(+AGGACA)GATCCTAGGTGGACACTTGGTACCTCTCTGT NGG 10 8 17 8 24 0 100 5 10
    240613131809_1orf CAGCAACAGAGAGGTACCAAGTGTCCACCTAG(+AGGACA)GATCCAGCCCGACGGCACCCTGACGTCTCCT NGG 10 8 17 8 24 0 100 5 10