Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613131837_3s0s ATAAGCTCCTGTCCCTGGGGACTAGGCATTAG(+AGGACA)GATCCCAGCATACTTGGGAGGCAGGGTCATT NGG 10 8 17 8 24 0 100 5 10
    240613131835_7ohq TGTGTAATGACCCTGCCTCCCAAGTATGCTGG(+AGGACA)GATCCTAATGCCTAGTCCCCAGGGACAGGAG NGG 10 8 17 8 24 0 100 5 10
    240613131834_d60q GGAAGCAGGCTGGCTCCGACCCAGGGCCTCAG(+AGGACA)GATCCCCTTGTGTACAGGGTCCATGAAACTG NGG 10 8 17 8 24 0 100 5 10
    240613131832_wv1x GTGGTCAGTTTCATGGACCCTGTACACAAGGG(+AGGACA)GATCCTGAGGCCCTGGGTCGGAGCCAGCCTG NGG 10 8 17 8 24 0 100 5 10
    240613131830_wp0g ACTTTGAGGCAGGCACAATGCTAGGAGCGCGG(+AGGACA)GATCCAGCAGCAGCCCCATACCCCCTCGGAT NGG 10 8 17 8 24 0 100 5 10
    240613131829_v15z GTACGATCCGAGGGGGTATGGGGCTGCTGCTG(+AGGACA)GATCCCGCGCTCCTAGCATTGTGCCTGCCTC NGG 10 8 17 8 24 0 100 5 10
    240613131828_vnol CGCTCTCTGCTTTGGCTTTAAAGTCAGGCCTG(+AGGACA)GTACCAGATAGGCAGGATCAGACTATCTTTG NGG 10 8 17 8 24 0 100 5 10
    240613131826_xdod GATGACAAAGATAGTCTGATCCTGCCTATCTG(+AGGACA)GTACCAGGCCTGACTTTAAAGCCAAAGCAGA NGG 10 8 17 8 24 0 100 5 10
    240613131825_xls0 TCCCTTCAACTTTCCCTAGTCTACATCTGCAG(+AGGACA)GTACCAATAAGACCATCTACATAACCTGCAA NGG 10 8 17 8 24 0 100 5 10
    240613131824_zlps GTTTCTTGCAGGTTATGTAGATGGTCTTATTG(+AGGACA)GTACCTGCAGATGTAGACTAGGGAAAGTTGA NGG 10 8 17 8 24 0 100 5 10