Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613131939_gafq CCCATCCTGTCAGGTTGGTGCGGAAGGGCTAG(+AGGACA)GATCCTTGCCGCCCTTCCCTTCGGTGCCGGA NGG 10 8 17 8 24 0 100 5 10
    240613131938_owz3 AGGGTTCCGGCACCGAAGGGAAGGGCGGCAAG(+AGGACA)GATCCTAGCCCTTCCGCACCAACCTGACAGG NGG 10 8 17 8 24 0 100 5 10
    240613131936_wq5j AGGCAGGTTCTCTCTGCTGTCCTCAGGCAGTG(+AGGACA)GTACCCTTGGGGGTCTCCTTGGTCTTCACAC NGG 10 8 17 8 24 0 100 5 10
    240613131934_nq45 CGTGTGTGTGAAGACCAAGGAGACCCCCAAGG(+AGGACA)GTACCACTGCCTGAGGACAGCAGAGAGAACC NGG 10 8 17 8 24 0 100 5 10
    240613131933_40iy GCCACGGCTTGCGATGTATAAGCACACAGAAG(+AGGACA)GATCCAGTGCAATAAAGACGTGAAACGTGTG NGG 10 8 17 8 24 0 100 5 10
    240613131932_l0e8 ACACACACACGTTTCACGTCTTTATTGCACTG(+AGGACA)GATCCTTCTGTGTGCTTATACATCGCAAGCC NGG 10 8 17 8 24 0 100 5 10
    240613131930_ogia TGAAACCACTCAAAGTCACAAAGAAACCCAGG(+AGGACA)GATCCCCTAAGCTGTGGTCCAGGTGCCCTAG NGG 10 8 17 8 24 0 100 5 10
    240613131927_w90r AAGTACTAGGGCACCTGGACCACAGCTTAGGG(+AGGACA)GATCCCTGGGTTTCTTTGTGACTTTGAGTGG NGG 10 8 17 8 24 0 100 5 10
    240613131926_y6ye CACTGGAGGTGCAGGTGCCTGGGCCAGCCTTG(+AGGACA)GATCCATATCCAGTATTTGGCAGATTCATGC NGG 10 8 17 8 24 0 100 5 10
    240613131925_zm75 GGCAGGCATGAATCTGCCAAATACTGGATATG(+AGGACA)GATCCAAGGCTGGCCCAGGCACCTGCACCTC NGG 10 8 17 8 24 0 100 5 10