Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613131952_hpkm GGACACCAGGTACTTTGCTCAAGGCTGGCCTG(+AGGACA)GATCCCATTCTTCCTAGGTCTTGACCTGCCC NGG 10 8 17 8 24 0 100 5 10
    240613131951_cd24 ATGTGGGGCAGGTCAAGACCTAGGAAGAATGG(+AGGACA)GATCCAGGCCAGCCTTGAGCAAAGTACCTGG NGG 10 8 17 8 24 0 100 5 10
    240613131950_r1u4 AAGGTCTAAATCTGCTCCACCCTCTGAGCGAG(+AGGACA)GTACCCTCACTTCAACCTGCCACACATTGCT NGG 10 8 17 8 24 0 100 5 10
    240613131948_wykv GTGCCAGCAATGTGTGGCAGGTTGAAGTGAGG(+AGGACA)GTACCTCGCTCAGAGGGTGGAGCAGATTTAG NGG 10 8 17 8 24 0 100 5 10
    240613131947_95f3 CCATGACTGCCAAGTCGCCTATTTTCTGTAAG(+AGGACA)GTACCGGCTCTCCACACAGAAACCTCCACTC NGG 10 8 17 8 24 0 100 5 10
    240613131945_d0yq CTTCGGAGTGGAGGTTTCTGTGTGGAGAGCCG(+AGGACA)GTACCTTACAGAAAATAGGCGACTTGGCAGT NGG 10 8 17 8 24 0 100 5 10
    240613131944_urb6 CCAGCTTGGCATTAATTACCAAGCCCGCTTTG(+AGGACA)GTACCCTGCTGGAGACCTGGCCTATGTACAG NGG 10 8 17 8 24 0 100 5 10
    240613131943_1vso GCTTGCTGTACATAGGCCAGGTCTCCAGCAGG(+AGGACA)GTACCAAAGCGGGCTTGGTAATTAATGCCAA NGG 10 8 17 8 24 0 100 5 10
    240613131941_6tko TTATGGCTGCGGCGTGCTGGCGTAGAACATTG(+AGGACA)GATCCGCCCCTAAGGTACTTGCGTGCCCGAC NGG 10 8 17 8 24 0 100 5 10
    240613131940_izjx GGGGCGTCGGGCACGCAAGTACCTTAGGGGCG(+AGGACA)GATCCAATGTTCTACGCCAGCACGCCGCAGC NGG 10 8 17 8 24 0 100 5 10