Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613132153_vj6s CTTGTGGCTCACTCCTGCTACTGAGACGGCTG(+AGGACA)GTACCAAGCAGCCACTTCAGCCACAGTGCCC NGG 10 8 17 8 24 0 100 5 10
    240613132152_li7u AACATTCCAGGCCGTTCGGAAGGCATCACTGG(+AGGACA)GTACCAGACTTGAAACCAGTCTTCAGTAACA NGG 10 8 17 8 24 0 100 5 10
    240613132150_4env ACACTTGTTACTGAAGACTGGTTTCAAGTCTG(+AGGACA)GTACCCAGTGATGCCTTCCGAACGGCCTGGA NGG 10 8 17 8 24 0 100 5 10
    240613132149_i3yq CCGTGGGATTATAGGAAAACCAGGGCCTCTTG(+AGGACA)GTACCCTGTCCTCAGAGTGTGTGACAGCCAC NGG 10 8 17 8 24 0 100 5 10
    240613132148_ydbd TTACAGTGGCTGTCACACACTCTGAGGACAGG(+AGGACA)GTACCAAGAGGCCCTGGTTTTCCTATAATCC NGG 10 8 17 8 24 0 100 5 10
    240613132146_gea1 GCACCTCCGAAGCTCTTAGTAATGTTGTGCTG(+AGGACA)GATCCTGTAAGTTCGCTCTGCAGTCACTCGC NGG 10 8 17 8 24 0 100 5 10
    240613132145_hgqw GGTTTGCGAGTGACTGCAGAGCGAACTTACAG(+AGGACA)GATCCAGCACAACATTACTAAGAGCTTCGGA NGG 10 8 17 8 24 0 100 5 10
    240613132143_lrdp TGATGCTCCACAAACTGTCTCATTAATCCATG(+AGGACA)GATCCTAAAACAAATTCTCTTTGTGTATTGT NGG 10 8 17 8 24 0 100 5 10
    240613132142_ft1e CACACACAATACACAAAGAGAATTTGTTTTAG(+AGGACA)GATCCATGGATTAATGAGACAGTTTGTGGAG NGG 10 8 17 8 24 0 100 5 10
    240613132140_psgi TCTTCCTTTTGAAGCTAGGCTGTGCTGTGTGG(+AGGACA)GATCCAGTGGTCTGTTCTTTACTGGAGGTAT NGG 10 8 17 8 24 0 100 5 10