Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613132138_34nc AACACATACCTCCAGTAAAGAACAGACCACTG(+AGGACA)GATCCCACACAGCACAGCCTAGCTTCAAAAG NGG 10 8 17 8 24 0 100 5 10
    240613132137_ozjm AGTAAAACCTTTAAACATGGTGACTATCTTCG(+AGGACA)GATCCCAAGGAGAATATTAACAGGCACCAGG NGG 10 8 17 8 24 0 100 5 10
    240613132135_11gy GAGTTCCTGGTGCCTGTTAATATTCTCCTTGG(+AGGACA)GATCCGAAGATAGTCACCATGTTTAAAGGTT NGG 10 8 17 8 24 0 100 5 10
    240613132134_irxd GTAAAAAAAAAAAAAAAGGCAGATGGCAGATG(+AGGACA)GATCCTGGAGCTTTTTCTCTCTTTTTTTTTC NGG 10 8 17 8 24 0 100 5 10
    240613132132_lgyg CTGGGGAAAAAAAAAGAGAGAAAAAGCTCCAG(+AGGACA)GATCCATCTGCCATCTGCCTTTTTTTTTTTT NGG 10 8 17 8 24 0 100 5 10
    240613132129_tswf CGAAGAAAAGTGCCTCTGTGTACTCTCCCTCG(+AGGACA)GATCCAGTTTTACCCTCAGCTGCATGAAAAC NGG 10 8 17 8 24 0 100 5 10
    240613132128_r0ca CAAACGTTTTCATGCAGCTGAGGGTAAAACTG(+AGGACA)GATCCGAGGGAGAGTACACAGAGGCACTTTT NGG 10 8 17 8 24 0 100 5 10
    240613132126_h95w AGTGTGAGCAACCACTTCTGTGTTTACCTGGG(+AGGACA)GATCCATCCCACAATCAGCCTCCAAACGCTG NGG 10 8 17 8 24 0 100 5 10
    240613132124_k7am GGTGTCAGCGTTTGGAGGCTGATTGTGGGATG(+AGGACA)GATCCCCAGGTAAACACAGAAGTGGTTGCTC NGG 10 8 17 8 24 0 100 5 10
    240613132121_ysql GGATGGACCATCCAGAGACTGCCCCACCCGGG(+AGGACA)GATCCATCCCATAATCAGCCACCAAATGCAG NGG 10 8 17 8 24 0 100 5 10