Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613132255_oevj CAGCACAATGGAGGGGGGAAGAAGTGTAAGAG(+AGGACA)GATCCTCTGATATCAAAAGGCTCTGCCATTT NGG 10 8 17 8 24 0 100 5 10
    240613132254_qqv7 TACATAGTTCAGAGTTGCTTCTGGGGGATCTG(+AGGACA)GTACCTTTAATATTTAAACTTTGAGCAATAT NGG 10 8 17 8 24 0 100 5 10
    240613132252_euje GCAGAATATTGCTCAAAGTTTAAATATTAAAG(+AGGACA)GTACCAGATCCCCCAGAAGCAACTCTGAACT NGG 10 8 17 8 24 0 100 5 10
    240613132251_yh04 CCCTTGCAGATAATGTTATGCCCTTGAGATAG(+AGGACA)GTACCCAATGCAAATTAAATGAATGTAAAAT NGG 10 8 17 8 24 0 100 5 10
    240613132250_jry3 TCAGTATTTTACATTCATTTAATTTGCATTGG(+AGGACA)GTACCTATCTCAAGGGCATAACATTATCTGC NGG 10 8 17 8 24 0 100 5 10
    240613132248_jn5w GTACATAACGTCCTTCAGTCATATTCATCCAG(+AGGACA)GTACCCTCCTTTGTCCCAGTCCCACCCCTCT NGG 10 8 17 8 24 0 100 5 10
    240613132246_n85e GGATAAGAGGGGTGGGACTGGGACAAAGGAGG(+AGGACA)GTACCTGGATGAATATGACTGAAGGACGTTA NGG 10 8 17 8 24 0 100 5 10
    240613132245_c1dc AGTGGCTCGTTAAGCTTTCCTTTTACCAGTTG(+AGGACA)GTACCAACTTTTAGCATTTCATAAAGTTTGT NGG 10 8 17 8 24 0 100 5 10
    240613132243_wetw ATTCTACAAACTTTATGAAATGCTAAAAGTTG(+AGGACA)GTACCAACTGGTAAAAGGAAAGCTTAACGAG NGG 10 8 17 8 24 0 100 5 10
    240613132242_o65r GTCCAGATGTGCGGCAGCCGTGGAGCTTCAGG(+AGGACA)GTACCCTGGAGCTCACAGGCTCTCTGGGAGT NGG 10 8 17 8 24 0 100 5 10