Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607030329_d0u0 GAGTCAATAATGTGAGGGTGGTGGGGTGGGGG(+ATATAT)GATCCTCAGTACAAGAAAACTGAATAGTGTT NGG 10 8 17 8 24 0 100 5 10
    240607030328_9icd TCCAAAACACTATTCAGTTTTCTTGTACTGAG(+ATATAT)GATCCCCCCACCCCACCACCCTCACATTATT NGG 10 8 17 8 24 0 100 5 10
    240607030327_h75m AAGGGTCTCTCACTAAAACTGAAGCTACGTTG(+ATATAT)GTACCCCAGAACGCCCCAGTCGTTCTCTTGC NGG 10 8 17 8 24 0 100 5 10
    240607030325_i53j AACGGGCAAGAGAACGACTGGGGCGTTCTGGG(+ATATAT)GTACCAACGTAGCTTCAGTTTTAGTGAGAGA NGG 10 8 17 8 24 0 100 5 10
    240607030324_q2fe CTCAGTTGGCAGAGTGCTAGCCTAGATGCATG(+ATATAT)GATCCTTGGGCTCAGGCCTCAGCACTGAACT NGG 10 8 17 8 24 0 100 5 10
    240607030321_rayh TGCCTAGTTCAGTGCTGAGGCCTGAGCCCAAG(+ATATAT)GATCCATGCATCTAGGCTAGCACTCTGCCAA NGG 10 8 17 8 24 0 100 5 10
    240607030319_hg2w CCACCCCACCACAGAATGCTGATATGCCAGTG(+ATATAT)GTACCCAGTACCTCTTGTATATAGTTATTGC NGG 10 8 17 8 24 0 100 5 10
    240607030317_0otk TATTTGCAATAACTATATACAAGAGGTACTGG(+ATATAT)GTACCACTGGCATATCAGCATTCTGTGGTGG NGG 10 8 17 8 24 0 100 5 10
    240607030316_nhyu ATGCTGTTCACATGGTGGGTATAACTTTCAAG(+ATATAT)GTACCACCCTGGCCTGCACCTGCCGGTCAGG NGG 10 8 17 8 24 0 100 5 10
    240607030314_w0yh TGGGTCCTGACCGGCAGGTGCAGGCCAGGGTG(+ATATAT)GTACCTTGAAAGTTATACCCACCATGTGAAC NGG 10 8 17 8 24 0 100 5 10