Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607030242_120g GGGATGGATGAAGTCGGTGCTGGCCATGCCTG(+ATATAT)GTACCATGATACTGGTTCTGGGCCAGAGAGG NGG 10 8 17 8 24 0 100 5 10
    240607030241_7syk AGCCACCTCTCTGGCCCAGAACCAGTATCATG(+ATATAT)GTACCAGGCATGGCCAGCACCGACTTCATCC NGG 10 8 17 8 24 0 100 5 10
    240607030240_5kc3 GGCTCTCACATCTATGAGATGGGGCTAAAATG(+ATATAT)GTACCTGTCCCTGTAGACTAACGGTATGAGG NGG 10 8 17 8 24 0 100 5 10
    240607030239_kz7z TTAATCCTCATACCGTTAGTCTACAGGGACAG(+ATATAT)GTACCATTTTAGCCCCATCTCATAGATGTGA NGG 10 8 17 8 24 0 100 5 10
    240607030237_7c2e CCTGAAAGACTACTTGCTCTCTGCTATAGAAG(+ATATAT)GATCCAGCACCCCTGTTCCCCACTTATAACT NGG 10 8 17 8 24 0 100 5 10
    240607030236_hngx CTGTAAGTTATAAGTGGGGAACAGGGGTGCTG(+ATATAT)GATCCTTCTATAGCAGAGAGCAAGTAGTCTT NGG 10 8 17 8 24 0 100 5 10
    240607030235_fh2r CACTGTCTCTCCCCCTCCCCCCAGGTAAATTG(+ATATAT)GATCCTGCTCTGTGGGGGGCAGGGGAGTAAT NGG 10 8 17 8 24 0 100 5 10
    240607030234_wr8v CTCTTATTACTCCCCTGCCCCCCACAGAGCAG(+ATATAT)GATCCAATTTACCTGGGGGGAGGGGGAGAGA NGG 10 8 17 8 24 0 100 5 10
    240607030233_wonj GTCAGCTGCAGGGGGATCTGGGTAAGGAAAAG(+ATATAT)GTACCGCTCTCCACAATCTCCTGTCTTTAAG NGG 10 8 17 8 24 0 100 5 10
    240607030231_as9x TCCAACTTAAAGACAGGAGATTGTGGAGAGCG(+ATATAT)GTACCTTTTCCTTACCCAGATCCCCCTGCAG NGG 10 8 17 8 24 0 100 5 10