Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607030312_k89q CTCTTCAAGCCCTCCTTTCAGTGGGGCCCTGG(+ATATAT)GTACCATGATGGCCTCCCTGTCTTCTAGGCA NGG 10 8 17 8 24 0 100 5 10
    240607030310_gpmw GACACTGCCTAGAAGACAGGGAGGCCATCATG(+ATATAT)GTACCCAGGGCCCCACTGAAAGGAGGGCTTG NGG 10 8 17 8 24 0 100 5 10
    240607030309_ymru ACCCTAAAGTTTGGAATGGGAACATGTGCATG(+ATATAT)GATCCAACAACCAACCCAACCCTCAAGTTCC NGG 10 8 17 8 24 0 100 5 10
    240607030308_6o59 GGACCGGAACTTGAGGGTTGGGTTGGTTGTTG(+ATATAT)GATCCATGCACATGTTCCCATTCCAAACTTT NGG 10 8 17 8 24 0 100 5 10
    240607030306_m61b GGCCACCGACACTGGGTAGGGATAGCCACGGG(+ATATAT)GTACCTGGTGTAATGTCCCTACCAGCTGACT NGG 10 8 17 8 24 0 100 5 10
    240607030304_m44x CTCTCAGTCAGCTGGTAGGGACATTACACCAG(+ATATAT)GTACCCCGTGGCTATCCCTACCCAGTGTCGG NGG 10 8 17 8 24 0 100 5 10
    240607030303_y57b CATAACAACTGCAGGTGCTCAGACCACTGTTG(+ATATAT)GTACCAGAACCAGTGCCTTGGTGGGGCCATC NGG 10 8 17 8 24 0 100 5 10
    240607030302_c4fq GGCAAGATGGCCCCACCAAGGCACTGGTTCTG(+ATATAT)GTACCAACAGTGGTCTGAGCACCTGCAGTTG NGG 10 8 17 8 24 0 100 5 10
    240607030300_03f1 TACTGTACTGGGTTCCGTGTGTCACTGGGGTG(+ATATAT)GTACCTATATTTTAATACTGTTCCTAACATT NGG 10 8 17 8 24 0 100 5 10
    240607030259_322q AATGAAATGTTAGGAACAGTATTAAAATATAG(+ATATAT)GTACCACCCCAGTGACACACGGAACCCAGTA NGG 10 8 17 8 24 0 100 5 10