Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607030258_5he1 CTTCCATGAACTCTTTAGGCCATGATTCTGTG(+ATATAT)GTACCTAGTTATGATAGTACAAATCAGATCA NGG 10 8 17 8 24 0 100 5 10
    240607030257_euy9 TTCTCTGATCTGATTTGTACTATCATAACTAG(+ATATAT)GTACCACAGAATCATGGCCTAAAGAGTTCAT NGG 10 8 17 8 24 0 100 5 10
    240607030255_3h9p AGTTTTTACACCATGGAAATGGGGTACACGGG(+ATATAT)GTACCCAGGTCATCAGCATGCCCTCTAAAGT NGG 10 8 17 8 24 0 100 5 10
    240607030253_brb7 TGGGTACTTTAGAGGGCATGCTGATGACCTGG(+ATATAT)GTACCCCGTGTACCCCATTTCCATGGTGTAA NGG 10 8 17 8 24 0 100 5 10
    240607030251_d3iv GGGACAAAGGGAAAAAAAAAACCAATTCAGAG(+ATATAT)GTACCCATATGCAGAGGTCAGCCTACAACAC NGG 10 8 17 8 24 0 100 5 10
    240607030250_b43t TGAATGTGTTGTAGGCTGACCTCTGCATATGG(+ATATAT)GTACCTCTGAATTGGTTTTTTTTTTCCCTTT NGG 10 8 17 8 24 0 100 5 10
    240607030248_wdft TGTCAAAGCATCCCCGAACTTCCTGCCCTAGG(+ATATAT)GATCCAGGAGGTAGCCATCCAGGAGGAGGTT NGG 10 8 17 8 24 0 100 5 10
    240607030246_747c ATGACAACCTCCTCCTGGATGGCTACCTCCTG(+ATATAT)GATCCCTAGGGCAGGAAGTTCGGGGATGCTT NGG 10 8 17 8 24 0 100 5 10
    240607030245_p9tz AGTTTAATTGGCTAAGCCCTTCAGCCCTTTAG(+ATATAT)GTACCTCATTAAAATGGAGATCTGTCTTGAG NGG 10 8 17 8 24 0 100 5 10
    240607030244_ahw5 AGAGTCTCAAGACAGATCTCCATTTTAATGAG(+ATATAT)GTACCTAAAGGGCTGAAGGGCTTAGCCAATT NGG 10 8 17 8 24 0 100 5 10