Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613183432_dwqo TACAGAGCAAGGCCCAGAGGAAGGGAGATGGG(+AGGACA)GATCCCTAAGAGAGTTGACTATATATAGACC NGG 10 8 17 8 24 0 100 5 10
    240613183430_96ba AAGTTCTTGTTCTCTGAGTCAGAGCTGGGTGG(+AGGACA)GTACCACTGTGCTGGTACTGAAAGGCGTGGG NGG 10 8 17 8 24 0 100 5 10
    240613183429_h87j CCTGCCCCACGCCTTTCAGTACCAGCACAGTG(+AGGACA)GTACCCACCCAGCTCTGACTCAGAGAACAAG NGG 10 8 17 8 24 0 100 5 10
    240613183427_o8j7 TAGAGACTGCCAACAGTGGGAGCTACTCCCTG(+AGGACA)GATCCCTCTTGGGCCGAGTAGTTTTTAAAGC NGG 10 8 17 8 24 0 100 5 10
    240613183425_zrdu ATTTGGCTTTAAAAACTACTCGGCCCAAGAGG(+AGGACA)GATCCAGGGAGTAGCTCCCACTGTTGGCAGT NGG 10 8 17 8 24 0 100 5 10
    240613183424_4hfo TGGTGGAACCTGTACACTGAAGAGTTAGTGAG(+AGGACA)GTACCCCAAATTACAGCAGTACCCCCTTCTC NGG 10 8 17 8 24 0 100 5 10
    240613183422_eqbm CTCCAGAGAAGGGGGTACTGCTGTAATTTGGG(+AGGACA)GTACCTCACTAACTCTTCAGTGTACAGGTTC NGG 10 8 17 8 24 0 100 5 10
    240613183421_djs7 CAATATCACGACGTGGAAATAGGAAGTTTGAG(+AGGACA)GATCCCCAGAGCAGCCAGCCTTCACGGGTTC NGG 10 8 17 8 24 0 100 5 10
    240613183419_hot2 CCCAGGAACCCGTGAAGGCTGGCTGCTCTGGG(+AGGACA)GATCCTCAAACTTCCTATTTCCACGTCGTGA NGG 10 8 17 8 24 0 100 5 10
    240613183417_qb6r AGGCTACGTGCAGTATCTGCAATTCACGGATG(+AGGACA)GATCCATCACTTTGGGTCTGAGGTACGGCTA NGG 10 8 17 8 24 0 100 5 10