Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613183347_eqi5 TGTCCTCTCTTCCACCCCGGTCCAAGAAAAAG(+AGGACA)GATCCTCAAGGCAGAGATGAGAAGGAAGCAA NGG 10 8 17 8 24 0 100 5 10
    240613183345_g3qx ATTCAGTCAGATGATGGCAAGAGGCTGCTGGG(+AGGACA)GTACCACATGACACAGCTAATGTTTAAATAA NGG 10 8 17 8 24 0 100 5 10
    240613183343_hue5 TATTATTATTTAAACATTAGCTGTGTCATGTG(+AGGACA)GTACCCCAGCAGCCTCTTGCCATCATCTGAC NGG 10 8 17 8 24 0 100 5 10
    240613183342_9m9w ATAAAAGCAGACTGGTACTAGCGGCTCTCAAG(+AGGACA)GATCCCCTTCTCATCCATTACAGAAGTTTGA NGG 10 8 17 8 24 0 100 5 10
    240613183340_qos4 TTTACTCAAACTTCTGTAATGGATGAGAAGGG(+AGGACA)GATCCTTGAGAGCCGCTAGTACCAGTCTGCT NGG 10 8 17 8 24 0 100 5 10
    240613183338_sdmx GTTCCTGCAGTATCGTGATGCCCTGAGATGGG(+AGGACA)GATCCGTGGGAACATGCAGTGCCCTGCAGTG NGG 10 8 17 8 24 0 100 5 10
    240613183335_2jhq CAGGGCACTGCAGGGCACTGCATGTTCCCACG(+AGGACA)GATCCCCATCTCAGGGCATCACGATACTGCA NGG 10 8 17 8 24 0 100 5 10
    240613183334_vnt3 TTGAACACTAGAGGGTTTCTTTCTAAGAGATG(+AGGACA)GTACCTAACCCTGTAACCAAGGAACTGTTTG NGG 10 8 17 8 24 0 100 5 10
    240613183333_ckvq TTTTCCAAACAGTTCCTTGGTTACAGGGTTAG(+AGGACA)GTACCATCTCTTAGAAAGAAACCCTCTAGTG NGG 10 8 17 8 24 0 100 5 10
    240613183332_0tsl TTCAACCCAAGGAAAAAGGTTGTGATTATTAG(+AGGACA)GTACCAGGCGTAAATAGCAAGGAAGAAATGT NGG 10 8 17 8 24 0 100 5 10