Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613183416_4wck ATGTTTAGCCGTACCTCAGACCCAAAGTGATG(+AGGACA)GATCCATCCGTGAATTGCAGATACTGCACGT NGG 10 8 17 8 24 0 100 5 10
    240613183415_ivzh GTACAATGGTGGCTTTAGCAACCACAAAGCTG(+AGGACA)GATCCAGAAGTACTCATTAGTGTTGTATTTG NGG 10 8 17 8 24 0 100 5 10
    240613183413_qhdo AGATGCAAATACAACACTAATGAGTACTTCTG(+AGGACA)GATCCAGCTTTGTGGTTGCTAAAGCCACCAT NGG 10 8 17 8 24 0 100 5 10
    240613183412_h0vt GAAGGAAGAGGATACGGATCACAAGGTCTCTG(+AGGACA)GATCCATGGATTTCTGACTGCCTTGTCTAAA NGG 10 8 17 8 24 0 100 5 10
    240613183410_mg83 ACAGCTTTAGACAAGGCAGTCAGAAATCCATG(+AGGACA)GATCCAGAGACCTTGTGATCCGTATCCTCTT NGG 10 8 17 8 24 0 100 5 10
    240613183408_18k0 AAAAATCAAAGCACTCATTCACTTGGGGGGAG(+AGGACA)GATCCTACGCAGGGGGGACTTGGTGCGTATA NGG 10 8 17 8 24 0 100 5 10
    240613183407_kxhm CTTAATATACGCACCAAGTCCCCCCTGCGTAG(+AGGACA)GATCCTCCCCCCAAGTGAATGAGTGCTTTGA NGG 10 8 17 8 24 0 100 5 10
    240613183405_zrjc GAGAAGGCCCTGTCTCCTCCTCAAGCCAAAGG(+AGGACA)GATCCCAGCAGCAGCATCGTGGTGGCATCTT NGG 10 8 17 8 24 0 100 5 10
    240613183404_yh8o CTCCAAAGATGCCACCACGATGCTGCTGCTGG(+AGGACA)GATCCCTTTGGCTTGAGGAGGAGACAGGGCC NGG 10 8 17 8 24 0 100 5 10
    240613183403_8mmj ACACACACACACACACACACTCTTAGCAGAAG(+AGGACA)GTACCTCATAGTCTTTCCTAGTCGTCTCATA NGG 10 8 17 8 24 0 100 5 10