Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613183315_zckh ACCACCACGCGGGTGGTGGGCATCGAACCTGG(+AGGACA)GTACCCTGGAAGAGCAGCCAGTGCTCTTAAC NGG 10 8 17 8 24 0 100 5 10
    240613183313_q48f TAACAGTTAAGAGCACTGGCTGCTCTTCCAGG(+AGGACA)GTACCCAGGTTCGATGCCCACCACCCGCGTG NGG 10 8 17 8 24 0 100 5 10
    240613183312_8rp7 AGATGATCAAAGCACTGCAGGAATTAGGTGTG(+AGGACA)GTACCCAGGGCCTGTAACCACAGTCAACTAG NGG 10 8 17 8 24 0 100 5 10
    240613183309_sfsi TCTAGCTAGTTGACTGTGGTTACAGGCCCTGG(+AGGACA)GTACCACACCTAATTCCTGCAGTGCTTTGAT NGG 10 8 17 8 24 0 100 5 10
    240613183308_dhxe TGCTGGAAGCCCTGGGTTTCACCTCCAGAACG(+AGGACA)GTACCTGTGATCACAACACTCAAGAGGTAGA NGG 10 8 17 8 24 0 100 5 10
    240613183307_9k2z CTGCCTCTACCTCTTGAGTGTTGTGATCACAG(+AGGACA)GTACCGTTCTGGAGGTGAAACCCAGGGCTTC NGG 10 8 17 8 24 0 100 5 10
    240613183305_wjs1 CACTATCACCGACATTCCTTCCAGCCACACTG(+AGGACA)GATCCAGGAAACCTGTGCTGTTACAGCTAAA NGG 10 8 17 8 24 0 100 5 10
    240613183303_5uyb GAACATTTAGCTGTAACAGCACAGGTTTCCTG(+AGGACA)GATCCAGTGTGGCTGGAAGGAATGTCGGTGA NGG 10 8 17 8 24 0 100 5 10
    240613183301_u5mc AGAAATACAGAAATGGAAGTCAAACCGCCGAG(+AGGACA)GATCCTGCGGCGGTGACCTCGGGGCTCTGGA NGG 10 8 17 8 24 0 100 5 10
    240613183259_mpit CTTCGTCCAGAGCCCCGAGGTCACCGCCGCAG(+AGGACA)GATCCTCGGCGGTTTGACTTCCATTTCTGTA NGG 10 8 17 8 24 0 100 5 10