Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613183242_ulwr TTTTGGGATAGCCACCCCCTGCAGTTGTTCAG(+AGGACA)GATCCACATGAAGACCAAGCTGCACATCTGA NGG 10 8 17 8 24 0 100 5 10
    240613183241_ow9z ATGTATCAGATGTGCAGCTTGGTCTTCATGTG(+AGGACA)GATCCTGAACAACTGCAGGGGGTGGCTATCC NGG 10 8 17 8 24 0 100 5 10
    240613183239_v29o TGGTCCAGTCTAGCTAGCCAGCTTGTTTGGAG(+AGGACA)GATCCCCATCTCTCCTTAGAAAGAGATTTAA NGG 10 8 17 8 24 0 100 5 10
    240613183237_eu4b AAATGTTAAATCTCTTTCTAAGGAGAGATGGG(+AGGACA)GATCCTCCAAACAAGCTGGCTAGCTAGACTG NGG 10 8 17 8 24 0 100 5 10
    240613183236_19y0 GGGGCTGTCTCTGACACCGTTGCCTGTCTTTG(+AGGACA)GATCCTCTTTCCCTAGCTGGACTTCCTGGTT NGG 10 8 17 8 24 0 100 5 10
    240613183235_gjbi GGCCCAACCAGGAAGTCCAGCTAGGGAAAGAG(+AGGACA)GATCCAAAGACAGGCAACGGTGTCAGAGACA NGG 10 8 17 8 24 0 100 5 10
    240613183234_ufus GTCAGTACTCACTCACCAGACTCGGGCAGAAG(+AGGACA)GATCCTAAGTTCAAAACCAGCCTGGAATACG NGG 10 8 17 8 24 0 100 5 10
    240613183232_rz92 TACTACGTATTCCAGGCTGGTTTTGAACTTAG(+AGGACA)GATCCTTCTGCCCGAGTCTGGTGAGTGAGTA NGG 10 8 17 8 24 0 100 5 10
    240613183231_2paz TCATGACTACAGCTCTGGGTCACCTCCTGTGG(+AGGACA)GATCCACCAGGGGACGGGTTACATCTTCCTG NGG 10 8 17 8 24 0 100 5 10
    240613183229_k908 GGCTTCAGGAAGATGTAACCCGTCCCCTGGTG(+AGGACA)GATCCCACAGGAGGTGACCCAGAGCTGTAGT NGG 10 8 17 8 24 0 100 5 10