Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613183155_bl8w GTCTGCCTTCAGCTCCCAACCCTAACCTCCTG(+AGGACA)GATCCCCAAGCCATGTTTCTCCCACAGGTGG NGG 10 8 17 8 24 0 100 5 10
    240613183153_em2k TGCATCCACCTGTGGGAGAAACATGGCTTGGG(+AGGACA)GATCCAGGAGGTTAGGGTTGGGAGCTGAAGG NGG 10 8 17 8 24 0 100 5 10
    240613183151_jno5 CACAGAGCATCTCAGTTGGACTGGACCACCAG(+AGGACA)GATCCCCAGAGCCAGCCTTCCCCACCTCCCC NGG 10 8 17 8 24 0 100 5 10
    240613183149_abhw GGACAGGGGAGGTGGGGAAGGCTGGCTCTGGG(+AGGACA)GATCCTGGTGGTCCAGTCCAACTGAGATGCT NGG 10 8 17 8 24 0 100 5 10
    240613183147_ivr8 GGGGGTCCGTGCAAGTGCTAACATATCTCCAG(+AGGACA)GATCCTCGGTGGTGCTAACCTATCTCCATGT NGG 10 8 17 8 24 0 100 5 10
    240613183145_k9bb GAGAAACATGGAGATAGGTTAGCACCACCGAG(+AGGACA)GATCCTGGAGATATGTTAGCACTTGCACGGA NGG 10 8 17 8 24 0 100 5 10
    240613183143_c75n AGAAATCTGGGTAAAGAGGAGAGAGATATGGG(+AGGACA)GTACCCTCCTCCCCTCACTGCTTGTGCCCTC NGG 10 8 17 8 24 0 100 5 10
    240613183141_sqhu GAGATGAGGGCACAAGCAGTGAGGGGAGGAGG(+AGGACA)GTACCCCATATCTCTCTCCTCTTTACCCAGA NGG 10 8 17 8 24 0 100 5 10
    240613183139_7f74 GTGAGGTGGGAGAAGTGTGTATACCCCTCCTG(+AGGACA)GATCCTGAATGTTTCTGCCTTTCCTTTGGGG NGG 10 8 17 8 24 0 100 5 10
    240613183134_n5pi CCCTCCCCCAAAGGAAAGGCAGAAACATTCAG(+AGGACA)GATCCAGGAGGGGTATACACACTTCTCCCAC NGG 10 8 17 8 24 0 100 5 10