Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240626063656_myn4 TTGACGCGGTTCTATCTAGTTACGCGTTAAACCAACTAGAAAgg(-TAATTTCTcCTaTTGgAGATACTGGTATTGCGGAGCCACGTTTTT)acgtTAATTTCTcCTaTTGgAGAT NGG 10 8 17 8 24 0 100 5 10
    240626061445_q27k TTGACGCGGTTCTATCTAGTTACcgGTTAAACCAACTAGAAAgg(-TAATTTCTcCTaTTGgAGATACTGcTATTGCtGAGaCACGTTTTT)TAATTTCTcCTaTTGgAGAT NGG 10 8 17 8 24 0 100 5 10
    240626060346_iwmj TTGACGCGGTTCTATCTAGTTACGCGTTAAACCAACTAGAAAggTAATTTCTcCTaTTGgAGAT(-ACTGGTATTGCGGAGCCACGTTTTT)TAATTTCTcCTaTTGgAGAT NGG 5 8 17 8 24 0 100 5 10
    240626054915_a7pa TTGACGCGGTTCTATCTAGTTACGCGTTAAACCAACTAGAAAGGTAATTTCTCCTATTGGAGAT(-CCTGCTATTGCTGAGCAACGTTTTT)TAATTTCTCCT NGG 30 5 17 5 24 0 100 5 1
    240626054408_35pu TTGACGCGGTTCTATCTAGTTACGCGTTAAACCAACTAGAAAggTAATTTCTcCTaTTGgAGAT(-ACTGGTATTGCGGAGCCACGTTTTT)TAATTTCTcCT NGG 10 10 17 10 24 0 100 8 10
    240626054325_cqzg TTGACGCGGTTCTATCTAGTTACGCGTTAAACCAACTAGAAAggTAATTTCTcCTaTTGgAGAT(-ACTGGTATTGCGGAGCCACGTTTTT)TAATTTCTcCT NGG 10 8 17 8 24 0 100 5 10
    240625210244_yu8v TGGCCTGGCTGGAAAAAGGTGTAAATACCTCCGTCATGCTGGAAAATCTCCGCCAAAGCTTATCCCTTCCCTCGATGCTTCGGGATGCTGCAATTGGCACTACCCCTTTCTCTACTTGCTCGGTGGGGACTTGGTTTACTCCTTCAGCACCACAGGAAAAGAGTACAAACACATCCCAGACAGGCCTGGTTGGCACCAAGC(/G)ACAGTACTTCTGAGACAGAGCAGCTCCTGTGTGGGTAAGCGCCCCTATGTCCTTGCACCTCGTGAGTTCCATTACCCTGGACCTTCCCCTCCCATCCCTTCCCCTCCTTTTCTTTTCGTCATGTTCTTCTCTTTCTGCTGCAGCCGGCCTCCAGATCTGACTGCCTTGTCTCGACATGACTTGGAAGATAACCTGCTGA NGG 10 8 17 8 24 0 100 5 10
    240625203538_3c3m CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC NGG 10 8 17 8 24 0 100 5 10
    240625165725_s42d ccacatccacagcatctataatgccagcctcaagaaatattttctcattaccttcttcctg(/T)ttcagcttcgccatcggattttatctgctgctcaagggactgggtgtagacctcctgtgg NGG 10 8 17 8 24 0 100 5 10
    240624195427_mw9e CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC NGG 10 8 17 8 24 0 100 5 10