Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613182606_fbe9 AATGTGCAGGACTTAAATACTCCTCTGCAGAG(+AGGACA)GATCCCCAAATGTTTTATAAAGAGCATTTTC NGG 10 8 17 8 24 0 100 5 10
    240613182559_0yux CATGAGAAAATGCTCTTTATAAAACATTTGGG(+AGGACA)GATCCTCTGCAGAGGAGTATTTAAGTCCTGC NGG 10 8 17 8 24 0 100 5 10
    240613182554_tgj6 TCTGAGGGAAGCACTCACGGAGTGTTTGTAAG(+AGGACA)GATCCAGGACAGACTTTACGACTTTTCAGTT NGG 10 8 17 8 24 0 100 5 10
    240613182548_fm7q GTTAAAACTGAAAAGTCGTAAAGTCTGTCCTG(+AGGACA)GATCCTTACAAACACTCCGTGAGTGCTTCCC NGG 10 8 17 8 24 0 100 5 10
    240613182542_xn8n GCCGTGGCTGCTCCGGAGTCTCCTCTGAGGTG(+AGGACA)GTACCTCTCAGAAACTCACACCTGGGACTCC NGG 10 8 17 8 24 0 100 5 10
    240613182536_wbb9 GGTGAGGAGTCCCAGGTGTGAGTTTCTGAGAG(+AGGACA)GTACCACCTCAGAGGAGACTCCGGAGCAGCC NGG 10 8 17 8 24 0 100 5 10
    240613182530_291w TATCTCCCCAGCTCAGTTGTGGATTTTCTAAG(+AGGACA)GTACCTGATACTATTTATTTAAGTATTTATT NGG 10 8 17 8 24 0 100 5 10
    240613182524_ibi1 TCATAAATAAATACTTAAATAAATAGTATCAG(+AGGACA)GTACCTTAGAAAATCCACAACTGAGCTGGGG NGG 10 8 17 8 24 0 100 5 10
    240613182516_pom3 TGAACAGAAACCTGACCGGTTGGAGGGCCCGG(+AGGACA)GATCCCTGCTGGCAGAGGCGGGCTTGAGTAA NGG 10 8 17 8 24 0 100 5 10
    240613182510_zpmf CCCCTTTACTCAAGCCCGCCTCTGCCAGCAGG(+AGGACA)GATCCCGGGCCCTCCAACCGGTCAGGTTTCT NGG 10 8 17 8 24 0 100 5 10