Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613182505_g7th GGTCTCCAAGAACAGTCCCCTGACAATCTAGG(+AGGACA)GATCCGGGCGGAGTCTGTCTTGGAAGGGACA NGG 10 8 17 8 24 0 100 5 10
    240613182458_ejmr AACTGTGTCCCTTCCAAGACAGACTCCGCCCG(+AGGACA)GATCCCTAGATTGTCAGGGGACTGTTCTTGG NGG 10 8 17 8 24 0 100 5 10
    240613182451_03xn TCAAGGAGAAAGAGGACCCTTGACTCTCCTGG(+AGGACA)GTACCAGATAAGTCAGGGACACTGTTTTGGT NGG 10 8 17 8 24 0 100 5 10
    240613182444_szyt TTCACACCAAAACAGTGTCCCTGACTTATCTG(+AGGACA)GTACCCAGGAGAGTCAAGGGTCCTCTTTCTC NGG 10 8 17 8 24 0 100 5 10
    240613182438_kc49 CAGCTTGGCTCCTGCTCCCTTGCAACTGAGTG(+AGGACA)GATCCAGTTTCCTCACAAATTGAACTGACAA NGG 10 8 17 8 24 0 100 5 10
    240613182432_skgi AGGTGTTGTCAGTTCAATTTGTGAGGAAACTG(+AGGACA)GATCCACTCAGTTGCAAGGGAGCAGGAGCCA NGG 10 8 17 8 24 0 100 5 10
    240613182426_nfgl AGGTAAACAAGGGAAGGACTGATTTCCTGTTG(+AGGACA)GATCCAGCAAACAGTGATATGATCCTCACAC NGG 10 8 17 8 24 0 100 5 10
    240613182416_1fy4 TTCATGTGTGAGGATCATATCACTGTTTGCTG(+AGGACA)GATCCAACAGGAAATCAGTCCTTCCCTTGTT NGG 10 8 17 8 24 0 100 5 10
    240613182412_kvnv ACTCCACATGTATGCACACGATGCAGGAATTG(+AGGACA)GATCCAGTTCAGGAGTTCAAGCTCTGTCCAG NGG 10 8 17 8 24 0 100 5 10
    240613182409_1f4g TGAAGCTGGACAGAGCTTGAACTCCTGAACTG(+AGGACA)GATCCAATTCCTGCATCGTGTGCATACATGT NGG 10 8 17 8 24 0 100 5 10