Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613182349_bpl5 TGCCTGACGGAGCTTTGGGCGCAGCTGGAAAG(+AGGACA)GATCCGTAGAGCAGTGGCTTCCCTTGTATGC NGG 10 8 17 8 24 0 100 5 10
    240613182347_y9a6 TGCACGCATACAAGGGAAGCCACTGCTCTACG(+AGGACA)GATCCTTTCCAGCTGCGCCCAAAGCTCCGTC NGG 10 8 17 8 24 0 100 5 10
    240613182346_6u2l GGTGCCTCTCTCCCCCCTTAGACATCAGACAG(+AGGACA)GATCCCTTTATAGAATAGCCAAGTAAGAAAC NGG 10 8 17 8 24 0 100 5 10
    240613182344_7uo4 CACTGGTTTCTTACTTGGCTATTCTATAAAGG(+AGGACA)GATCCTGTCTGATGTCTAAGGGGGGAGAGAG NGG 10 8 17 8 24 0 100 5 10
    240613182343_zq0a CAGCACTGCTCCCTGGGTGCTGAGCACTGGTG(+AGGACA)GATCCAGTGCCCCAGAGGGCAAGTCCCTTCG NGG 10 8 17 8 24 0 100 5 10
    240613182341_nin7 AGCTGCGAAGGGACTTGCCCTCTGGGGCACTG(+AGGACA)GATCCACCAGTGCTCAGCACCCAGGGAGCAG NGG 10 8 17 8 24 0 100 5 10
    240613182338_7utz AAAACCATCAAGATGCAATGGTCTTTATTAAG(+AGGACA)GTACCTGCGGTCAGCTGGCAACCATCTCTTG NGG 10 8 17 8 24 0 100 5 10
    240613182332_yup1 TGTTTCAAGAGATGGTTGCCAGCTGACCGCAG(+AGGACA)GTACCTTAATAAAGACCATTGCATCTTGATG NGG 10 8 17 8 24 0 100 5 10
    240613182325_e6nh GTAGCGTCTCTGGAGGCCAGAAGATGGCATTG(+AGGACA)GATCCCCGGAACTGGAGTCACAAGTTGTGAG NGG 10 8 17 8 24 0 100 5 10
    240613182317_gvly TGGAGCTCACAACTTGTGACTCCAGTTCCGGG(+AGGACA)GATCCAATGCCATCTTCTGGCCTCCAGAGAC NGG 10 8 17 8 24 0 100 5 10