Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613182406_8sd7 AACACAACCTGGCTCTCTGTCTCTGTTCCTGG(+AGGACA)GTACCAGCAAATTGGAAGGTGTATTTTGACA NGG 10 8 17 8 24 0 100 5 10
    240613182405_9blg GTCTGTGTCAAAATACACCTTCCAATTTGCTG(+AGGACA)GTACCCAGGAACAGAGACAGAGAGCCAGGTT NGG 10 8 17 8 24 0 100 5 10
    240613182404_425v GAAGAACCTCTTTTCCAACCTCAAGTCTCTTG(+AGGACA)GATCCTAAGGTCTAGCCAGCCTGACCTTGAC NGG 10 8 17 8 24 0 100 5 10
    240613182402_k2os TGGTCGTCAAGGTCAGGCTGGCTAGACCTTAG(+AGGACA)GATCCAAGAGACTTGAGGTTGGAAAAGAGGT NGG 10 8 17 8 24 0 100 5 10
    240613182400_ffxt AAAACTACTTCTGATGCTGGGTCCTGAACCAG(+AGGACA)GTACCTCGTACCTACTGCACATAATGGCACC NGG 10 8 17 8 24 0 100 5 10
    240613182358_q6oz TTGGTGGTGCCATTATGTGCAGTAGGTACGAG(+AGGACA)GTACCTGGTTCAGGACCCAGCATCAGAAGTA NGG 10 8 17 8 24 0 100 5 10
    240613182357_742k CAGCACCTTAATGATCGCCGTTGGCCACGTGG(+AGGACA)GATCCCCAATGCAAACATATTTATAGGTGGA NGG 10 8 17 8 24 0 100 5 10
    240613182355_0wmc CGCACTCCACCTATAAATATGTTTGCATTGGG(+AGGACA)GATCCCACGTGGCCAACGGCGATCATTAAGG NGG 10 8 17 8 24 0 100 5 10
    240613182353_2x8f GTACAGCAGTCAGACTAGAACTGAACGTCGGG(+AGGACA)GATCCAGGGCGCGGGCAGTGGAAACGCAGGC NGG 10 8 17 8 24 0 100 5 10
    240613182350_w8iu TCCCTGCCTGCGTTTCCACTGCCCGCGCCCTG(+AGGACA)GATCCCCGACGTTCAGTTCTAGTCTGACTGC NGG 10 8 17 8 24 0 100 5 10