Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613182816_ks62 TGGCCTACGATGTTCTTCAGTCCCTGTTCTGG(+AGGACA)GATCCTGGGGCTTATCTTGGGCACAGAAGGC NGG 10 8 17 8 24 0 100 5 10
    240613182809_5kgj TCTTAGCCTTCTGTGCCCAAGATAAGCCCCAG(+AGGACA)GATCCCAGAACAGGGACTGAAGAACATCGTA NGG 10 8 17 8 24 0 100 5 10
    240613182803_ir9g TTGGCTCTGAGACCTGGAGCGCGTGCTCAGTG(+AGGACA)GATCCCCGGGCAGGTGGGCCTCTGTGGCTCA NGG 10 8 17 8 24 0 100 5 10
    240613182756_jaym GGGTCTGAGCCACAGAGGCCCACCTGCCCGGG(+AGGACA)GATCCACTGAGCACGCGCTCCAGGTCTCAGA NGG 10 8 17 8 24 0 100 5 10
    240613182744_d44d TCCCACCTGAGGGTCTGGCGTGTTTCCATCTG(+AGGACA)GATCCCTTTCTGCAGAGGTTCACAGGCCCAT NGG 10 8 17 8 24 0 100 5 10
    240613182738_kqe3 TAGACATGGGCCTGTGAACCTCTGCAGAAAGG(+AGGACA)GATCCAGATGGAAACACGCCAGACCCTCAGG NGG 10 8 17 8 24 0 100 5 10
    240613182732_yyzs CGGGGGGGGGGCGGGAGGACAGGGACTCTATG(+AGGACA)GTACCCGGAGGGGCGGGGCAGAGAGGGAGGG NGG 10 8 17 8 24 0 100 5 10
    240613182725_vas6 ATAGCCCCTCCCTCTCTGCCCCGCCCCTCCGG(+AGGACA)GTACCATAGAGTCCCTGTCCTCCCGCCCCCC NGG 10 8 17 8 24 0 100 5 10
    240613182717_eyse GGATGAGCTCAGTTGGCGTCTGCTCGCCTTGG(+AGGACA)GTACCAGCCTTGGAAAACGCTGTCGCCCCAA NGG 10 8 17 8 24 0 100 5 10
    240613182712_wndg AGCGTTTGGGGCGACAGCGTTTTCCAAGGCTG(+AGGACA)GTACCCAAGGCGAGCAGACGCCAACTGAGCT NGG 10 8 17 8 24 0 100 5 10