Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607021727_8yzi TAAAAAAATATATTGCTTGGTACCAGACAGAG(+ATATAT)GATCCAGGAAGGGGAAATGGTTGTGAACAAA NGG 10 8 17 8 24 0 100 5 10
    240607021726_ycwx CACCCTGGCTCTAGCACCATCTCCTGGGCTAG(+ATATAT)GATCCTGGGCTAAATAAAAAGAAAGAAGACT NGG 10 8 17 8 24 0 100 5 10
    240607021724_838j TCCTAAGTCTTCTTTCTTTTTATTTAGCCCAG(+ATATAT)GATCCTAGCCCAGGAGATGGTGCTAGAGCCA NGG 10 8 17 8 24 0 100 5 10
    240607021721_a0ay TCCTCAGAAAATTGGACATAGTACTACCAGAG(+ATATAT)GATCCTGCAATACCTCTCCTGGGCATAGATC NGG 10 8 17 8 24 0 100 5 10
    240607021720_2lou TTCTGGATCTATGCCCAGGAGAGGTATTGCAG(+ATATAT)GATCCTCTGGTAGTACTATGTCCAATTTTCT NGG 10 8 17 8 24 0 100 5 10
    240607021718_yqwi GGATGGACCATCTAGAGACTGCCATATCCACG(+ATATAT)GATCCATCCCATAATTAGCCTCCAAACGATG NGG 10 8 17 8 24 0 100 5 10
    240607021717_q3by GGTGTCATCGTTTGGAGGCTAATTATGGGATG(+ATATAT)GATCCGTGGATATGGCAGTCTCTAGATGGTC NGG 10 8 17 8 24 0 100 5 10
    240607021715_z96o CATCTTCATGAGTTGCTCTGTTATTTTGCAGG(+ATATAT)GATCCTGGGCAGCTTTGGCATTATCATGCAC NGG 10 8 17 8 24 0 100 5 10
    240607021713_twix GGAGTGTGCATGATAATGCCAAAGCTGCCCAG(+ATATAT)GATCCCTGCAAAATAACAGAGCAACTCATGA NGG 10 8 17 8 24 0 100 5 10
    240607021711_w94q GCAACCCAATAATACTATCATAATCCGGACAG(+ATATAT)GATCCTCCCCGACTGTGGAGAGAGACCCTGG NGG 10 8 17 8 24 0 100 5 10