Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607021653_rlii ACCCCATGGTGGCTTACAACCATCTGTAATGG(+ATATAT)GATCCAATACCTTCTTCTGTCCTACAGGTGT NGG 10 8 17 8 24 0 100 5 10
    240607021652_aovg AGTGGCCCTGGGCAGTAGCTCTTAGGGGGTGG(+ATATAT)GTACCTCGACAGTGGATTTCAGCTTGAGTGA NGG 10 8 17 8 24 0 100 5 10
    240607021650_fhqk AGATGTCACTCAAGCTGAAATCCACTGTCGAG(+ATATAT)GTACCCACCCCCTAAGAGCTACTGCCCAGGG NGG 10 8 17 8 24 0 100 5 10
    240607021649_4ki8 TGCTGAGATGGTGATGTTACTCTCCATTTTTG(+ATATAT)GTACCAGTTCTGAGACTGTAAGACTTTTCAT NGG 10 8 17 8 24 0 100 5 10
    240607021648_w115 CCCAGATGAAAAGTCTTACAGTCTCAGAACTG(+ATATAT)GTACCAAAAATGGAGAGTAACATCACCATCT NGG 10 8 17 8 24 0 100 5 10
    240607021647_oplp TTTTTGTGAGGGAGTTGATCCTGGGCAGTTAG(+ATATAT)GTACCCTGGAAGGAGGGGAGGGAACCTCGAT NGG 10 8 17 8 24 0 100 5 10
    240607021645_yyzp GCTTCATCGAGGTTCCCTCCCCTCCTTCCAGG(+ATATAT)GTACCTAACTGCCCAGGATCAACTCCCTCAC NGG 10 8 17 8 24 0 100 5 10
    240607021643_okiq ACCGGACCTTGTGGCCAAGTAGGATTCTGAGG(+ATATAT)GATCCAGACGAGAAGCCCAAAGAGTTCAGAT NGG 10 8 17 8 24 0 100 5 10
    240607021642_so7y CCGGCATCTGAACTCTTTGGGCTTCTCGTCTG(+ATATAT)GATCCCTCAGAATCCTACTTGGCCACAAGGT NGG 10 8 17 8 24 0 100 5 10
    240607021641_ksi2 AGTCCTTAGCAGCCCCCACCACCCACGCATCG(+ATATAT)GATCCCATTCTCAACCAGGACCCCCGCACCT NGG 10 8 17 8 24 0 100 5 10