Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607021610_qyta GCTTCCTGATAAAACTGCCACAGACCACAGAG(+ATATAT)GATCCTTGACAAAGCATTCTGGAAAATAGAG NGG 10 8 17 8 24 0 100 5 10
    240607021608_4t76 AGGACAGAAGAGGAGAAAGGGAAGGCCTGAGG(+ATATAT)GTACCTCAGCCGCCAGCTACCTTCACTTTGA NGG 10 8 17 8 24 0 100 5 10
    240607021607_yx6n AGACTTCAAAGTGAAGGTAGCTGGCGGCTGAG(+ATATAT)GTACCCTCAGGCCTTCCCTTTCTCCTCTTCT NGG 10 8 17 8 24 0 100 5 10
    240607021605_upz7 GTAAACAGCAAGAGGCTTTATTGGGAACACGG(+ATATAT)GTACCCGGGCGACTCAGTCTATCGGAGGACT NGG 10 8 17 8 24 0 100 5 10
    240607021603_0jom GCGCCAGTCCTCCGATAGACTGAGTCGCCCGG(+ATATAT)GTACCCGTGTTCCCAATAAAGCCTCTTGCTG NGG 10 8 17 8 24 0 100 5 10
    240607021602_thgc TTATGCAGATGCTCTTGCTGTGTTCCTCAAAG(+ATATAT)GATCCAAGCCATAAAATTCTTTATGCATGTC NGG 10 8 17 8 24 0 100 5 10
    240607021600_50pg ACTGTGACATGCATAAAGAATTTTATGGCTTG(+ATATAT)GATCCTTTGAGGAACACAGCAAGAGCATCTG NGG 10 8 17 8 24 0 100 5 10
    240607021559_ly2o AATGAAGTTCACTGTGTTTATAAACTGAAAAG(+ATATAT)GTACCGGGTCTTACAGCATTTTATATATCAC NGG 10 8 17 8 24 0 100 5 10
    240607021557_zx4b AAGGTGTGATATATAAAATGCTGTAAGACCCG(+ATATAT)GTACCTTTTCAGTTTATAAACACAGTGAACT NGG 10 8 17 8 24 0 100 5 10
    240607021555_51ey GGTTCGGTGCCTCCCCTTCCCCAGCAGCCAAG(+ATATAT)GATCCTGGGGCCCTGTACAATATAGATGCTC NGG 10 8 17 8 24 0 100 5 10