Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607021710_3qu3 TTTCTCCAGGGTCTCTCTCCACAGTCGGGGAG(+ATATAT)GATCCTGTCCGGATTATGATAGTATTATTGG NGG 10 8 17 8 24 0 100 5 10
    240607021708_67ar TTGGTAGTTCATTCGAAAGTTGAGCTCATCCG(+ATATAT)GTACCGGGCTGGTTTCCTATGAATGAGTTCA NGG 10 8 17 8 24 0 100 5 10
    240607021706_86q7 CTCAGTGAACTCATTCATAGGAAACCAGCCCG(+ATATAT)GTACCGGATGAGCTCAACTTTCGAATGAACT NGG 10 8 17 8 24 0 100 5 10
    240607021704_c4je GGGGCCCCCTGCCCGCGCCCAGACACTTGATG(+ATATAT)GATCCCCACCAACGCCCCCCCTACCCCCACC NGG 10 8 17 8 24 0 100 5 10
    240607021702_evgc GAGGTGGTGGGGGTAGGGGGGGCGTTGGTGGG(+ATATAT)GATCCATCAAGTGTCTGGGCGCGGGCAGGGG NGG 10 8 17 8 24 0 100 5 10
    240607021701_97oi CTCTGGACCACTGAGCCATCTCCAACTTCTTG(+ATATAT)GATCCTTGGGACAACAAAAGGAGAGGCTGCC NGG 10 8 17 8 24 0 100 5 10
    240607021658_tdg0 AGAGTGGCAGCCTCTCCTTTTGTTGTCCCAAG(+ATATAT)GATCCAAGAAGTTGGAGATGGCTCAGTGGTC NGG 10 8 17 8 24 0 100 5 10
    240607021657_w26j AGCGAACGCGTGTAAGACACTACTACACTCAG(+ATATAT)GATCCACAAGCTGTTGATTCCAAATTCCAGA NGG 10 8 17 8 24 0 100 5 10
    240607021656_b5ya CTCAGTCTGGAATTTGGAATCAACAGCTTGTG(+ATATAT)GATCCTGAGTGTAGTAGTGTCTTACACGCGT NGG 10 8 17 8 24 0 100 5 10
    240607021655_kmxo TATGGACACCTGTAGGACAGAAGAAGGTATTG(+ATATAT)GATCCCATTACAGATGGTTGTAAGCCACCAT NGG 10 8 17 8 24 0 100 5 10