Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607021639_6aha TGGACAGGTGCGGGGGTCCTGGTTGAGAATGG(+ATATAT)GATCCGATGCGTGGGTGGTGGGGGCTGCTAA NGG 10 8 17 8 24 0 100 5 10
    240607021637_hf8i GTCATCTTGTCAGTTCCTAACTGGAACCTTGG(+ATATAT)GATCCTTCTTCTTCCTCTCTTTCTTTCTTTC NGG 10 8 17 8 24 0 100 5 10
    240607021636_obu9 AGAAAGAAAGAAAGAAAGAGAGGAAGAAGAAG(+ATATAT)GATCCCAAGGTTCCAGTTAGGAACTGACAAG NGG 10 8 17 8 24 0 100 5 10
    240607021634_71tf CTGATACTTGATGTTCCAGCCCAGCACCTCTG(+ATATAT)GATCCTTGAAGTACCCATGATGGGTAATAGG NGG 10 8 17 8 24 0 100 5 10
    240607021633_yum9 AAAGGCCTATTACCCATCATGGGTACTTCAAG(+ATATAT)GATCCAGAGGTGCTGGGCTGGAACATCAAGT NGG 10 8 17 8 24 0 100 5 10
    240607021632_glka ACTCTTCCGAAGTTCTCACTCCTCCTGAACAG(+ATATAT)GATCCCCTGACCCGTTAGGAGCCTGCTTCGT NGG 10 8 17 8 24 0 100 5 10
    240607021629_031m TGTGCACGAAGCAGGCTCCTAACGGGTCAGGG(+ATATAT)GATCCTGTTCAGGAGGAGTGAGAACTTCGGA NGG 10 8 17 8 24 0 100 5 10
    240607021628_4g6i TCCTTGCTCACCCAAACTAGATATATATTTGG(+ATATAT)GATCCATGGTTGTTCAGACTTTACATGAAGA NGG 10 8 17 8 24 0 100 5 10
    240607021626_998j TAAGATCTTCATGTAAAGTCTGAACAACCATG(+ATATAT)GATCCCAAATATATATCTAGTTTGGGTGAGC NGG 10 8 17 8 24 0 100 5 10
    240607021624_dywd TTTGAATCCAATGATCCAGCTCCAAGGATTGG(+ATATAT)GATCCAGTGAGCCCTCTCCAATCCAAACCTT NGG 10 8 17 8 24 0 100 5 10