Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607004411_j5wo CCCCCACAGCCCTTTGATGTTACCAGGCACCG(+ATATAT)GATCCCCATGGCTTCCGTCACCCCTGGTCCT NGG 10 8 17 8 24 0 100 5 10
    240607004409_h5zg TGGTAAGGACCAGGGGTGACGGAAGCCATGGG(+ATATAT)GATCCGGTGCCTGGTAACATCAAAGGGCTGT NGG 10 8 17 8 24 0 100 5 10
    240607004407_a4k7 TATTTTTCCCTTTCAAATAAAACACCAGTCAG(+ATATAT)GTACCTTTATCCCAGTCTTAACTGTCCCAAA NGG 10 8 17 8 24 0 100 5 10
    240607004406_fl3f CCAGATTTGGGACAGTTAAGACTGGGATAAAG(+ATATAT)GTACCTGACTGGTGTTTTATTTGAAAGGGAA NGG 10 8 17 8 24 0 100 5 10
    240607004404_a1p2 ACACCCACACTATATAAAGTACTTAGTAGGGG(+ATATAT)GTACCCCGTAGTGTGATTTAAGTCATTGGTG NGG 10 8 17 8 24 0 100 5 10
    240607004402_d6uv AATCTCACCAATGACTTAAATCACACTACGGG(+ATATAT)GTACCCCCTACTAAGTACTTTATATAGTGTG NGG 10 8 17 8 24 0 100 5 10
    240607004400_1dcv TGGCAAACCACTGTCTGCTATGGTTCTGAGAG(+ATATAT)GATCCAGAGCCTTGGTATTCAAAAGCCAAGT NGG 10 8 17 8 24 0 100 5 10
    240607004359_saae CCGACACTTGGCTTTTGAATACCAAGGCTCTG(+ATATAT)GATCCTCTCAGAACCATAGCAGACAGTGGTT NGG 10 8 17 8 24 0 100 5 10
    240607004357_rdzu TGAAGTCCATTGCAGCTACAGTTGCTGATGGG(+ATATAT)GATCCCATAGGAGAAAATGGACCAGGGAAGA NGG 10 8 17 8 24 0 100 5 10
    240607004356_flc7 CGTTCTCTTCCCTGGTCCATTTTCTCCTATGG(+ATATAT)GATCCCCATCAGCAACTGTAGCTGCAATGGA NGG 10 8 17 8 24 0 100 5 10