Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607004242_mt4d TAGTCTTGAGGCTGCCTTTGCCAAATGAAGAG(+ATATAT)GTACCAAGCAGTCATATGTAATTTTTTCCCT NGG 10 8 17 8 24 0 100 5 10
    240607004241_many GCAGCCGAGCATGAGCGTTACGGGAAGAGATG(+ATATAT)GATCCTTACCAGGTTGTGAGGCGGGAACGAC NGG 10 8 17 8 24 0 100 5 10
    240607004240_681d GAGCAGTCGTTCCCGCCTCACAACCTGGTAAG(+ATATAT)GATCCATCTCTTCCCGTAACGCTCATGCTCG NGG 10 8 17 8 24 0 100 5 10
    240607004239_n493 TTTTTTCCCCCACGATAGCATGTTTATTTTAG(+ATATAT)GATCCATTAAATCATACATCTTTTCAAGCTA NGG 10 8 17 8 24 0 100 5 10
    240607004238_geyr ATAGATAGCTTGAAAAGATGTATGATTTAATG(+ATATAT)GATCCTAAAATAAACATGCTATCGTGGGGGA NGG 10 8 17 8 24 0 100 5 10
    240607004236_9luo TGTTCAGAGAATGTATAGCAGGGGTTTGCCTG(+ATATAT)GTACCTCTACATTGTCATCTCAATATCCCCA NGG 10 8 17 8 24 0 100 5 10
    240607004235_ghoo CAACCTGGGGATATTGAGATGACAATGTAGAG(+ATATAT)GTACCAGGCAAACCCCTGCTATACATTCTCT NGG 10 8 17 8 24 0 100 5 10
    240607004233_bwtu AAGAACTTCAACTTTTGATAGGTTGCTAGCGG(+ATATAT)GTACCCCCCTTGTGAGGGACTTAAAGCATAA NGG 10 8 17 8 24 0 100 5 10
    240607004231_qj5q AAAATTTATGCTTTAAGTCCCTCACAAGGGGG(+ATATAT)GTACCCGCTAGCAACCTATCAAAAGTTGAAG NGG 10 8 17 8 24 0 100 5 10
    240607004230_jyc6 TTCTGGTTTCTGGAACTAGTGAGAGGCATGAG(+ATATAT)GATCCAAGAAAAAGGCAGAGAGCAAGTAGGT NGG 10 8 17 8 24 0 100 5 10