Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607004214_17dr CTTTTTATTAGTCACATTGCAGCTATCCTCAG(+ATATAT)GTACCAAATGTTTTTGATTTTTAAATAAAGA NGG 10 8 17 8 24 0 100 5 10
    240607004213_f06g GCAGCAGGTCCTACCCTGGAAGGACACAATAG(+ATATAT)GTACCCCTCACATATGTGAAAATAAATGGCA NGG 10 8 17 8 24 0 100 5 10
    240607004211_8v8n ACTATTGCCATTTATTTTCACATATGTGAGGG(+ATATAT)GTACCTATTGTGTCCTTCCAGGGTAGGACCT NGG 10 8 17 8 24 0 100 5 10
    240607004210_xgcy ACCTCTGTCCTCATCAGGCAGGAGAGGCAAGG(+ATATAT)GTACCTCAGGGCAGACACAGCCAGGCGCCTG NGG 10 8 17 8 24 0 100 5 10
    240607004208_zro2 CCTCACAGGCGCCTGGCTGTGTCTGCCCTGAG(+ATATAT)GTACCCTTGCCTCTCCTGCCTGATGAGGACA NGG 10 8 17 8 24 0 100 5 10
    240607004206_g0sc CAGACTGCCCTTGGCAGCAACCCACCCTAGGG(+ATATAT)GATCCCGTTCCTGACAAACTCCAGCCCCAGA NGG 10 8 17 8 24 0 100 5 10
    240607004204_kr9q AGGTCTCTGGGGCTGGAGTTTGTCAGGAACGG(+ATATAT)GATCCCCTAGGGTGGGTTGCTGCCAAGGGCA NGG 10 8 17 8 24 0 100 5 10
    240607004203_fwi6 CGGCCAACCTTACCTCATTACTGTGAAGCGAG(+ATATAT)GATCCTTGTTTGCACACGCTTTGACTGGATT NGG 10 8 17 8 24 0 100 5 10
    240607004202_rmyz TGTTAAATCCAGTCAAAGCGTGTGCAAACAAG(+ATATAT)GATCCTCGCTTCACAGTAATGAGGTAAGGTT NGG 10 8 17 8 24 0 100 5 10
    240607004200_fi9z AAAGAAGAAGCTGTAAGGAGAGTTGCCACTTG(+ATATAT)GTACCACCCCAGGCTGGACCCCTACAGCAGC NGG 10 8 17 8 24 0 100 5 10