Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607004115_2lv8 TTTTCTTGTGATGCTCCCCACAGCACTAGGTG(+ATATAT)GTACCTACTACACCTTCATTTTACAGGTAAG NGG 10 8 17 8 24 0 100 5 10
    240607004113_xwj8 GACATTTTCTACCACTTAATGTAACATGCCTG(+ATATAT)GTACCCGTCATGCAAAGCTGCAACACACAGC NGG 10 8 17 8 24 0 100 5 10
    240607004111_6wh8 AGTGGGCTGTGTGTTGCAGCTTTGCATGACGG(+ATATAT)GTACCAGGCATGTTACATTAAGTGGTAGAAA NGG 10 8 17 8 24 0 100 5 10
    240607004110_rbpp ACGCCCACCATTTATCTGAGTTTTTAAGGTAG(+ATATAT)GTACCTTATTCCAGCACTCAGAAAGAGGCAG NGG 10 8 17 8 24 0 100 5 10
    240607004109_2ec7 TTCTCCTGCCTCTTTCTGAGTGCTGGAATAAG(+ATATAT)GTACCTACCTTAAAAACTCAGATAAATGGTG NGG 10 8 17 8 24 0 100 5 10
    240607004107_jnay CACCCACACCTGTCTGTACCTCACTCCAGAGG(+ATATAT)GATCCACCGCCCTCTTCTGCCCTCTGCAGGC NGG 10 8 17 8 24 0 100 5 10
    240607004106_d2e0 CAGGTGCCTGCAGAGGGCAGAAGAGGGCGGTG(+ATATAT)GATCCCTCTGGAGTGAGGTACAGACAGGTGT NGG 10 8 17 8 24 0 100 5 10
    240607004103_twbm CAGCTTTCCTCTCTTTACCTTTCACGCCTTGG(+ATATAT)GATCCTAAGGATAAAGCCCTGGTTGGTAGTA NGG 10 8 17 8 24 0 100 5 10
    240607004101_vpmj TTAGTTACTACCAACCAGGGCTTTATCCTTAG(+ATATAT)GATCCCAAGGCGTGAAAGGTAAAGAGAGGAA NGG 10 8 17 8 24 0 100 5 10
    240607004059_pqnc AAAACGGCAGCACCTCACGGCTGTCTTTCTAG(+ATATAT)GATCCTCCATTATTCCTTAGAAGCAAGGACC NGG 10 8 17 8 24 0 100 5 10