Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607004041_fdbu TTGGACCTCAATGGCAGCATCCTGAGCGGTGG(+ATATAT)GTACCCTGGCTCGACGCCCCTCCAGCACAAC NGG 10 8 17 8 24 0 100 5 10
    240607004040_grs1 GAAAGAGACAAAAGCTGAACTATCCCTTAGTG(+ATATAT)GATCCCCACTTATGTGCCTGGGTGAGAAAGA NGG 10 8 17 8 24 0 100 5 10
    240607004038_4qxi AGAGTTCTTTCTCACCCAGGCACATAAGTGGG(+ATATAT)GATCCACTAAGGGATAGTTCAGCTTTTGTCT NGG 10 8 17 8 24 0 100 5 10
    240607004037_vy9m AAAAAAAAAAGTCCTGGTCTAATGATTATCTG(+ATATAT)GATCCAGCCTAATTCTCAGATTCTCAAGAAT NGG 10 8 17 8 24 0 100 5 10
    240607004035_jcwc TAGTGATTCTTGAGAATCTGAGAATTAGGCTG(+ATATAT)GATCCAGATAATCATTAGACCAGGACTTTTT NGG 10 8 17 8 24 0 100 5 10
    240607004034_bsyy AAGGTAGATGCCAAACACCAGCATCTCACTGG(+ATATAT)GATCCACAATCAGGAAATACTTCAGCACTGG NGG 10 8 17 8 24 0 100 5 10
    240607004033_2726 CCTGGCCAGTGCTGAAGTATTTCCTGATTGTG(+ATATAT)GATCCCAGTGAGATGCTGGTGTTTGGCATCT NGG 10 8 17 8 24 0 100 5 10
    240607004031_oyd8 TTTGCAGGAGTTGGTTCTCCTTGAACTGAGTG(+ATATAT)GATCCTAGGGATTAAACGCCTGTCACTGTGT NGG 10 8 17 8 24 0 100 5 10
    240607004028_ar56 CCTCAACACAGTGACAGGCGTTTAATCCCTAG(+ATATAT)GATCCACTCAGTTCAAGGAGAACCAACTCCT NGG 10 8 17 8 24 0 100 5 10
    240607004027_415e GACGGGCACGGTGAGCTAGGACAAGGAACGAG(+ATATAT)GATCCAGGAGTCTGTGTAGGAATTAGAAGAT NGG 10 8 17 8 24 0 100 5 10