Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607004058_eyx0 GACCAGGTCCTTGCTTCTAAGGAATAATGGAG(+ATATAT)GATCCTAGAAAGACAGCCGTGAGGTGCTGCC NGG 10 8 17 8 24 0 100 5 10
    240607004057_z86a GGCCCGTGTAGTTGGCTCAGTGTCACACAACG(+ATATAT)GTACCAGAGCACCATGATGCCTTTCTGTCTG NGG 10 8 17 8 24 0 100 5 10
    240607004055_nkcm GCGGTCAGACAGAAAGGCATCATGGTGCTCTG(+ATATAT)GTACCGTTGTGTGACACTGAGCCAACTACAC NGG 10 8 17 8 24 0 100 5 10
    240607004053_pdg1 CACCCTTGGGCCTACCTGTTTGCCTGAGGTGG(+ATATAT)GATCCCAGGAGCCTCACCCCTACCACCCCTC NGG 10 8 17 8 24 0 100 5 10
    240607004051_fvvo ACATGGAGGGGTGGTAGGGGTGAGGCTCCTGG(+ATATAT)GATCCCACCTCAGGCAAACAGGTAGGCCCAA NGG 10 8 17 8 24 0 100 5 10
    240607004050_oode GAGAGGAGGCCCTTGCCCTAGGCTGTGAGTAG(+ATATAT)GTACCCAGCTGGGCACAACTCAGGCTTCATC NGG 10 8 17 8 24 0 100 5 10
    240607004048_3lag AAAAGGATGAAGCCTGAGTTGTGCCCAGCTGG(+ATATAT)GTACCTACTCACAGCCTAGGGCAAGGGCCTC NGG 10 8 17 8 24 0 100 5 10
    240607004046_crtz ACACCAGGCTCCCCACGAGTTCCCATTGTGGG(+ATATAT)GATCCTGGCAGGATTCCTTTCTCTAAAATGG NGG 10 8 17 8 24 0 100 5 10
    240607004045_sdcv TGTTTCCATTTTAGAGAAAGGAATCCTGCCAG(+ATATAT)GATCCCCACAATGGGAACTCGTGGGGAGCCT NGG 10 8 17 8 24 0 100 5 10
    240607004042_hnkz CTGCAGTTGTGCTGGAGGGGCGTCGAGCCAGG(+ATATAT)GTACCCACCGCTCAGGATGCTGCCATTGAGG NGG 10 8 17 8 24 0 100 5 10