Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607004026_2jnn CACCCATCTTCTAATTCCTACACAGACTCCTG(+ATATAT)GATCCTCGTTCCTTGTCCTAGCTCACCGTGC NGG 10 8 17 8 24 0 100 5 10
    240607004025_2fo5 GAGAAGAAGGAGGAGGTACAGGGTGCTTTTTG(+ATATAT)GTACCAGGGAGAAAGTCATTGTGGGAAGAGG NGG 10 8 17 8 24 0 100 5 10
    240607004022_0osg AACTACCTCTTCCCACAATGACTTTCTCCCTG(+ATATAT)GTACCAAAAAGCACCCTGTACCTCCTCCTTC NGG 10 8 17 8 24 0 100 5 10
    240607004021_pkaz CCTGGGTTTGTTTGGTGAGGACCCTGTGGTTG(+ATATAT)GTACCTTAGCTTGGAACAGACCTGGATGAAC NGG 10 8 17 8 24 0 100 5 10
    240607004019_ljdr ACACAGTTCATCCAGGTCTGTTCCAAGCTAAG(+ATATAT)GTACCAACCACAGGGTCCTCACCAAACAAAC NGG 10 8 17 8 24 0 100 5 10
    240607004017_j38r GGCTTTCCTCAGCATGGCCACATCAGCCATGG(+ATATAT)GTACCTGGAAATGAAGCTCCAGAGCAGGTCC NGG 10 8 17 8 24 0 100 5 10
    240607004015_5hn5 GGCAGGGACCTGCTCTGGAGCTTCATTTCCAG(+ATATAT)GTACCCATGGCTGATGTGGCCATGCTGAGGA NGG 10 8 17 8 24 0 100 5 10
    240607004013_pvqa CACACCAAGAAGTCCATCCTTACAGTCCCACG(+ATATAT)GTACCTCTGCGTTCTAGGACTGTAGGACTGT NGG 10 8 17 8 24 0 100 5 10
    240607004012_2qyp ATGTCACAGTCCTACAGTCCTAGAACGCAGAG(+ATATAT)GTACCGTGGGACTGTAAGGATGGACTTCTTG NGG 10 8 17 8 24 0 100 5 10
    240607004010_a7jl AGGAGACCAATGTCTGTAGACTGTGGCGACTG(+ATATAT)GATCCCTCCTAGGTTATGACTTTTCCCAAGT NGG 10 8 17 8 24 0 100 5 10