Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607004229_8pf5 AGTTAACCTACTTGCTCTCTGCCTTTTTCTTG(+ATATAT)GATCCTCATGCCTCTCACTAGTTCCAGAAAC NGG 10 8 17 8 24 0 100 5 10
    240607004228_12v5 AGATAGAAAAATTGTCAGTACACTAGCTCTAG(+ATATAT)GATCCAGAATGCAGGTGGCTGGTTTCACCCA NGG 10 8 17 8 24 0 100 5 10
    240607004226_wr0m CCCTTTGGGTGAAACCAGCCACCTGCATTCTG(+ATATAT)GATCCTAGAGCTAGTGTACTGACAATTTTTC NGG 10 8 17 8 24 0 100 5 10
    240607004225_18lf GGTTTCATACCATTTGAGCCAGTGTCATGGTG(+ATATAT)GTACCCATACTCTTTTGTTCAGCTCTCAGTC NGG 10 8 17 8 24 0 100 5 10
    240607004224_omlr GACAGGACTGAGAGCTGAACAAAAGAGTATGG(+ATATAT)GTACCACCATGACACTGGCTCAAATGGTATG NGG 10 8 17 8 24 0 100 5 10
    240607004223_534n AACAAAAGAGCTTTGGGCCAGTTTGACAGTTG(+ATATAT)GTACCCCCTCTAATGCCTCACCTAAATGGGG NGG 10 8 17 8 24 0 100 5 10
    240607004220_vz51 AAACGCCCCATTTAGGTGAGGCATTAGAGGGG(+ATATAT)GTACCAACTGTCAAACTGGCCCAAAGCTCTT NGG 10 8 17 8 24 0 100 5 10
    240607004219_8gyr AGATTGTTATCAAGGAAACTCAGTAAGAATAG(+ATATAT)GTACCCAAATAAATCGGGAAGTTTGTATCCT NGG 10 8 17 8 24 0 100 5 10
    240607004217_ybqj GGGCTAGGATACAAACTTCCCGATTTATTTGG(+ATATAT)GTACCTATTCTTACTGAGTTTCCTTGATAAC NGG 10 8 17 8 24 0 100 5 10
    240607004216_v2gl TACTATCTTTATTTAAAAATCAAAAACATTTG(+ATATAT)GTACCTGAGGATAGCTGCAATGTGACTAATA NGG 10 8 17 8 24 0 100 5 10